Pseudomonas aeruginosa PAO1, PA4201 (ddlA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4201
Name
ddlA
Replicon chromosome
Genomic location 4700906 - 4701946 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252890.1
GI 15599396
Affymetrix PA4201_ddlA_at
DNASU PaCD00007825
Entrez 879033
GenBank AAG07588.1
INSDC AAG07588.1
NCBI Locus Tag PA4201
protein_id(GenBank) gb|AAG07588.1|AE004836_7|gnl|PseudoCAP|PA4201
TIGR NTL03PA04201
UniParc UPI00000C5C6E
UniProtKB Acc Q9HWI0
UniProtKB ID DDLA_PSEAE
UniRef100 UniRef100_Q9HWI0
UniRef50 UniRef50_Q9HWI0
UniRef90 UniRef90_Q9HWI0

Product

Feature Type CDS
Coding Frame 1
Product Name
D-alanine-D-alanine ligase A
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.66
Kyte-Doolittle Hydrophobicity Value 0.054
Molecular Weight (kDa) 36.5
Isoelectric Point (pI) 4.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 596 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001506 (317 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4201
Search term: ddlA

Human Homologs

References

Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes.
Zawadzke LE, Bugg TD, Walsh CT
Biochemistry 1991 Feb 12;30(6):1673-82
PubMed ID: 1993184
D-alanine: D-alanine ligase of Escherichia coli. Expression, purification and inhibitory studies on the cloned enzyme.
al-Bar OA, O'Connor CD, Giles IG, Akhtar M
Biochem J 1992 Mar 15;282 ( Pt 3)(Pt 3):747-52
PubMed ID: 1554356
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant.
Fan C, Park IS, Walsh CT, Knox JR
Biochemistry 1997 Mar 4;36(9):2531-8
PubMed ID: 9054558