Pseudomonas aeruginosa PAO1, PA4216 (phzG1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4216
Name
phzG1
Replicon chromosome
Genomic location 4719419 - 4720063 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252906.1
GI 15599412
Entrez 881835
GenBank AAG07604.1
INSDC AAG07604.1
NCBI Locus Tag PA4216
protein_id(GenBank) gb|AAG07604.1|AE004838_8|gnl|PseudoCAP|PA4216
TIGR NTL03PA04217
UniParc UPI00000D4220
UniProtKB Acc G3XCV4
UniProtKB ID G3XCV4_PSEAE
UniRef100 UniRef100_Q820A5
UniRef50 UniRef50_Q51793
UniRef90 UniRef90_Q820A5

Product

Feature Type CDS
Coding Frame 1
Product Name
probable pyridoxamine 5'-phosphate oxidase
Product Name Confidence: Class 1
Synonyms probable phenazine biosynthesis protein
Evidence for Translation
Charge (pH 7) -1.86
Kyte-Doolittle Hydrophobicity Value -0.637
Molecular Weight (kDa) 24.3
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1T9M OXIDOREDUCTASE 05/18/04 X-ray crystal structure of phzG from pseudomonas aeruginosa Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Flavin mononucleotide DB03247 DrugBank 5.1.4 O69755 Phenazine biosynthesis protein 100.0 214 1.2e-122

Virulence Evidence

Source 1
Database VFDB
Category Phenazine biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 350 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001491 (444 members)
Putative Inparalogs 1 predicted inparalogs
probable pyridoxamine 5'-phosphate oxidase (PA1905) 100.0% identity

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4216
Search term: phzG1

Human Homologs

References

Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
J Bacteriol 2001 Nov;183(21):6454-65
PubMed ID: 11591691
Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84.
Pierson LS 3rd, Gaffney T, Lam S, Gong F
FEMS Microbiol Lett 1995 Dec 15;134(2-3):299-307
PubMed ID: 8586283