Pseudomonas aeruginosa PAO1, PA4230 (pchB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4230
Name	pchB
Replicon	chromosome
Genomic location	4744819 - 4745124 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_252920.1
GI	15599426
Affymetrix	PA4230_pchB_at
Entrez	881846
GenBank	AAG07618.1
INSDC	AAG07618.1
NCBI Locus Tag	PA4230
protein_id(GenBank)	gb|AAG07618.1|AE004840_4|gnl|PseudoCAP|PA4230
TIGR	NTL03PA04231
UniParc	UPI00001313E9
UniProtKB Acc	Q51507
UniProtKB ID	PCHB_PSEAE
UniRef100	UniRef100_Q51507
UniRef50	UniRef50_Q51507
UniRef90	UniRef90_Q51507

Product

Feature Type	CDS
Coding Frame	1
Product Name	salicylate biosynthesis protein PchB Product Name Confidence: Class 1
Synonyms	chorismate mutase isochorismate-pyruvate lyase
Evidence for Translation
Charge (pH 7)	-1.80
Kyte-Doolittle Hydrophobicity Value	-0.282
Molecular Weight (kDa)	11.4
Isoelectric Point (pI)	5.19

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3REM	LYASE	04/04/11	Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0
2H9D	LYASE	06/09/06	Pyruvate-Bound Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0
3HGX	LYASE	05/14/09	Crystal Structure of Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase K42A mutant in complex with salicylate and pyruvate	Pseudomonas aeruginosa	2.5	X-RAY DIFFRACTION	99.0
3RET	LYASE	04/05/11	Salicylate and Pyruvate Bound Structure of the Isochorismate-Pyruvate Lyase K42E Mutant from Pseudomonas aerugionsa	Pseudomonas aeruginosa	1.79	X-RAY DIFFRACTION	99.0
2H9C	LYASE	06/09/06	Native Crystal Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.35	X-RAY DIFFRACTION	100.0
3HGW	LYASE	05/14/09	Apo Structure of Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase I87T mutant	Pseudomonas aeruginosa	2.25	X-RAY DIFFRACTION	99.0

Virulence Evidence

Source 1
Database	VFDB : VF0095
Category	Pyochelin
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 62 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000704 (672 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4230 Search term: pchB Search term: salicylate biosynthesis protein PchB

Human Homologs

References