Pseudomonas aeruginosa PAO1, PA4292 (pitA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4292
Name
pitA
Replicon chromosome
Genomic location 4815043 - 4816512 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252982.1
GI 15599488
Affymetrix PA4292_at
Entrez 881583
GenBank AAG07680.1
INSDC AAG07680.1
NCBI Locus Tag PA4292
protein_id(GenBank) gb|AAG07680.1|AE004845_6|gnl|PseudoCAP|PA4292
TIGR NTL03PA04293
UniParc UPI00000C5C9D
UniProtKB Acc Q9HWA8
UniProtKB ID Q9HWA8_PSEAE
UniRef100 UniRef100_W0YYG2
UniRef50 UniRef50_P43676
UniRef90 UniRef90_A0A080VD11

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphate transporter, PitA
Product Name Confidence: Class 1
Synonyms phosphate transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (52.92 kDa, PMID:21751344)
Charge (pH 7) 6.58
Kyte-Doolittle Hydrophobicity Value 0.435
Molecular Weight (kDa) 52.9
Isoelectric Point (pI) 9.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 332 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000441 (1290 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4292
Search term: pitA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
PitA Controls the H2- and H3-T6SSs through PhoB in Pseudomonas aeruginosa.
Zhao X, Xu C, Qu J, Jin Y, Bai F, Cheng Z, Wu W, Pan X
Appl Environ Microbiol 2023 Jun 28;89(6):e0209422
PubMed ID: 37184394
Pseudomonas aeruginosa Phosphate Transporter PitA (PA4292) Controls Susceptibility to Aminoglycoside Antibiotics by Regulating the Proton Motive Force.
Zhao X, Jin Y, Bai F, Cheng Z, Wu W, Pan X
Antimicrob Agents Chemother 2022 Dec 20;66(12):e0099222
PubMed ID: 36346250
Mutation of PA4292 in Pseudomonas aeruginosa Increases β-Lactam Resistance through Upregulating Pyocyanin Production.
Zhao X, Jin Y, Bai F, Cheng Z, Wu W, Pan X
Antimicrob Agents Chemother 2022 Jul 19;66(7):e0042122
PubMed ID: 35695577