Pseudomonas aeruginosa PAO1, PA4314 (purU1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4314
Name
purU1
Replicon chromosome
Genomic location 4842701 - 4843552 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253004.1
GI 15599510
Affymetrix PA4314_purU1_at
DNASU PaCD00007869
Entrez 881557
GenBank AAG07702.1
INSDC AAG07702.1
NCBI Locus Tag PA4314
protein_id(GenBank) gb|AAG07702.1|AE004848_1|gnl|PseudoCAP|PA4314
TIGR NTL03PA04315
UniParc UPI00000C5CB2
UniProtKB Acc Q9HW87
UniProtKB ID Q9HW87_PSEAE
UniRef100 UniRef100_W0Z077
UniRef50 UniRef50_Q4K7B6
UniRef90 UniRef90_A6VAY3

Product

Feature Type CDS
Coding Frame 1
Product Name
formyltetrahydrofolate deformylase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.85
Kyte-Doolittle Hydrophobicity Value -0.233
Molecular Weight (kDa) 32.4
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 605 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003912 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4314
Search term: purU1

Human Homologs

References

purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis.
Nagy PL, McCorkle GM, Zalkin H
J Bacteriol 1993 Nov;175(21):7066-73
PubMed ID: 8226647
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994