Pseudomonas aeruginosa PAO1, PA4376 (pncB2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4376
Name
pncB2
Replicon chromosome
Genomic location 4907841 - 4909037 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253066.1
GI 15599572
Affymetrix PA4376_pncB2_at
Entrez 881378
GenBank AAG07764.1
INSDC AAG07764.1
NCBI Locus Tag PA4376
protein_id(GenBank) gb|AAG07764.1|AE004853_7|gnl|PseudoCAP|PA4376
TIGR NTL03PA04377
UniParc UPI0000131D30
UniProtKB Acc Q9HW26
UniProtKB ID PNCB2_PSEAE
UniRef100 UniRef100_Q9HW26
UniRef50 UniRef50_Q88DF7
UniRef90 UniRef90_Q9HW26

Product

Feature Type CDS
Coding Frame 1
Product Name
nicotinate phosphoribosyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.31
Kyte-Doolittle Hydrophobicity Value -0.113
Molecular Weight (kDa) 45.7
Isoelectric Point (pI) 6.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1YIR TRANSFERASE 01/12/05 Crystal Structure of a Nicotinate Phosphoribosyltransferase Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 201 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003966 (826 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4376
Search term: pncB2

Human Homologs

References

Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase.
Wubbolts MG, Terpstra P, van Beilen JB, Kingma J, Meesters HA, Witholt B
J Biol Chem 1990 Oct 15;265(29):17665-72
PubMed ID: 2211655
Cloning and nucleic acid sequence of the Salmonella typhimurium pncB gene and structure of nicotinate phosphoribosyltransferase.
Vinitsky A, Teng H, Grubmeyer CT
J Bacteriol 1991 Jan;173(2):536-40
PubMed ID: 1987148