Pseudomonas aeruginosa PAO1, PA4425

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4425
Name
Synonym: yraO
Replicon chromosome
Genomic location 4959926 - 4960519 (+ strand)
CommentModeling evidence for S7P-isomerase. BLAST of PA4425 against NCBI showed E = 2e-59 with Sedoheptulose 7-phosphate isomerase Ralstonia eutropha H16.

Cross-References

RefSeq NP_253115.1
GI 15599621
Affymetrix PA4425_at
Entrez 881196
GenBank AAG07813.1
INSDC AAG07813.1
NCBI Locus Tag PA4425
protein_id(GenBank) gb|AAG07813.1|AE004857_4|gnl|PseudoCAP|PA4425
TIGR NTL03PA04426
UniParc UPI000012E842
UniProtKB Acc Q9HVZ0
UniProtKB ID GMHA_PSEAE
UniRef100 UniRef100_B7UZK3
UniRef50 UniRef50_C1DQ86
UniRef90 UniRef90_Q4ZNX7

Product

Feature Type CDS
Coding Frame 1
Product Name
sedoheptulose 7-phosphate isomerase GmhA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.89
Kyte-Doolittle Hydrophobicity Value -0.023
Molecular Weight (kDa) 21.4
Isoelectric Point (pI) 4.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3BJZ ISOMERASE 12/05/07 Crystal structure of Pseudomonas aeruginosa phosphoheptose isomerase Pseudomonas aeruginosa PAO1 2.4 X-RAY DIFFRACTION 100.0
1X92 ISOMERASE 08/19/04 CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA PHOSPHOHEPTOSE ISOMERASE IN COMPLEX WITH REACTION PRODUCT D-GLYCERO-D-MANNOPYRANOSE-7-PHOSPHATE Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
D-Glycero-D-Mannopyranose-7-Phosphate DB02470 DrugBank 5.1.4 Q9HVZ0 Phosphoheptose isomerase 100.0 197 8.7e-104

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 350 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004015 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4425

Human Homologs

References

Involvement of the Haemophilus ducreyi gmhA gene product in lipooligosaccharide expression and virulence.
Bauer BA, Stevens MK, Hansen EJ
Infect Immun 1998 Sep;66(9):4290-8
PubMed ID: 9712780
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P, Valvano MA, Messner P
J Bacteriol 2002 Jan;184(2):363-9
PubMed ID: 11751812