Pseudomonas aeruginosa PAO1, PA4484 (gatB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4484
Name
gatB
Replicon chromosome
Genomic location 5015535 - 5016980 (+ strand)

Cross-References

RefSeq NP_253174.1
GI 15599680
Affymetrix PA4484_gatB_at
Entrez 881181
GenBank AAG07872.1
INSDC AAG07872.1
NCBI Locus Tag PA4484
protein_id(GenBank) gb|AAG07872.1|AE004862_6|gnl|PseudoCAP|PA4484
TIGR NTL03PA04485
UniParc UPI000012B15C
UniProtKB Acc Q9HVT7
UniProtKB ID GATB_PSEAE
UniRef100 UniRef100_B7V024
UniRef50 UniRef50_Q92QK5
UniRef90 UniRef90_B7V024

Product

Feature Type CDS
Coding Frame 1
Product Name
Glu-tRNA(Gln) amidotransferase subunit B
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.47
Kyte-Doolittle Hydrophobicity Value -0.347
Molecular Weight (kDa) 53.1
Isoelectric Point (pI) 5.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WJ3 LIGASE/RNA 09/29/14 Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 3.705 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 567 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004073 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4484
Search term: gatB

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutamyl-tRNA amidotransferase subunit B [Source:HGNC Symbol;Acc:HGNC:8849]
E-value: 3.2e-88
Percent Identity: 37.9

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994