Pseudomonas aeruginosa PAO1, PA4565 (proB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4565
Name
proB
Replicon chromosome
Genomic location 5113481 - 5114599 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253255.1
GI 15599761
Affymetrix PA4565_proB_at
Entrez 880909
GenBank AAG07953.1
INSDC AAG07953.1
NCBI Locus Tag PA4565
protein_id(GenBank) gb|AAG07953.1|AE004870_4|gnl|PseudoCAP|PA4565
TIGR NTL03PA04566
UniParc UPI00001322E0
UniProtKB Acc Q9HVL9
UniProtKB ID PROB_PSEAE
UniRef100 UniRef100_B7V0A8
UniRef50 UniRef50_Q3K6L0
UniRef90 UniRef90_B7V0A8

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamate 5-kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.87
Kyte-Doolittle Hydrophobicity Value -0.015
Molecular Weight (kDa) 39.8
Isoelectric Point (pI) 7.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 532 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004224 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4565
Search term: proB

Human Homologs

Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]
E-value: 4.4e-29
Percent Identity: 34.0
Ensembl 110, assembly GRCh38.p14
aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]
E-value: 4.4e-29
Percent Identity: 34.2

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
Deutch AH, Rushlow KE, Smith CJ
Nucleic Acids Res 1984 Aug 10;12(15):6337-55
PubMed ID: 6089111
Molecular cloning and sequence analysis of the proBA operon from an extremely thermophilic eubacterium Thermus thermophilus.
Kosuge T, Tabata K, Hoshino T
FEMS Microbiol Lett 1994 Oct 15;123(1-2):55-61
PubMed ID: 7988899