Pseudomonas aeruginosa PAO1, PA4569 (ispB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4569
Name
ispB
Synonym: cel
Replicon chromosome
Genomic location 5116865 - 5117833 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253259.1
GI 15599765
Affymetrix PA4569_ispB_at
DNASU PaCD00007979
Entrez 880946
GenBank AAG07957.1
INSDC AAG07957.1
NCBI Locus Tag PA4569
protein_id(GenBank) gb|AAG07957.1|AE004870_8|gnl|PseudoCAP|PA4569
TIGR NTL03PA04570
UniParc UPI00000C5D68
UniProtKB Acc Q9HVL5
UniProtKB ID Q9HVL5_PSEAE
UniRef100 UniRef100_W0YXY1
UniRef50 UniRef50_P0AD57
UniRef90 UniRef90_A0A024HND2

Product

Feature Type CDS
Coding Frame 1
Product Name
octaprenyl-diphosphate synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.15
Kyte-Doolittle Hydrophobicity Value 0.031
Molecular Weight (kDa) 34.9
Isoelectric Point (pI) 4.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 657 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004228 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4569
Search term: ispB

Human Homologs

Ensembl 110, assembly GRCh38.p14
decaprenyl diphosphate synthase subunit 1 [Source:HGNC Symbol;Acc:HGNC:17759]
E-value: 2.7e-30
Percent Identity: 28.3

References

The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase.
Asai K, Fujisaki S, Nishimura Y, Nishino T, Okada K, Nakagawa T, Kawamukai M, Matsuda H
Biochem Biophys Res Commun 1994 Jul 15;202(1):340-5
PubMed ID: 8037730