Pseudomonas aeruginosa PAO1, PA4661 (pagL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4661
Name
pagL
Replicon chromosome
Genomic location 5229459 - 5229980 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253350.1
GI 15599856
Affymetrix PA4661_at
Entrez 881344
GenBank AAG08048.1
INSDC AAG08048.1
NCBI Locus Tag PA4661
protein_id(GenBank) gb|AAG08048.1|AE004880_4|gnl|PseudoCAP|PA4661
TIGR NTL03PA04662
UniParc UPI00000C5DB5
UniProtKB Acc Q9HVD1
UniProtKB ID PAGL_PSEAE
UniRef100 UniRef100_Q9HVD1
UniRef50 UniRef50_Q9HVD1
UniRef90 UniRef90_Q9HVD1

Product

Feature Type CDS
Coding Frame 1
Product Name
Lipid A 3-O-deacylase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (18.39 kDa, PMID:21751344)
Detected using 2-DE, MALDI-TOF/MS analysis (18.39 kDa, pI 5.9, PMID:16335974)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.30
Kyte-Doolittle Hydrophobicity Value -0.042
Molecular Weight (kDa) 18.4
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Unknown Class 3
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2ERV MEMBRANE PROTEIN 10/25/05 Crystal structure of the outer membrane enzyme PagL Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 31 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004311 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4661
Search term: pagL

Human Homologs

References

Dissemination of lipid A deacylases (pagL) among gram-negative bacteria: identification of active-site histidine and serine residues.
Geurtsen J, Steeghs L, Hove JT, van der Ley P, Tommassen J
J Biol Chem 2005 Mar 4;280(9):8248-59
PubMed ID: 15611102
The Pseudomonas aeruginosa lipid A deacylase: selection for expression and loss within the cystic fibrosis airway.
Ernst RK, Adams KN, Moskowitz SM, Kraig GM, Kawasaki K, Stead CM, Trent MS, Miller SI
J Bacteriol 2006 Jan;188(1):191-201
PubMed ID: 16352835
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344