Pseudomonas aeruginosa PAO1, PA4674

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4674
Name
Synonym: vapI
Replicon chromosome
Genomic location 5242254 - 5242559 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253363.1
GI 15599869
Affymetrix PA4674_at
DNASU PaCD00008030
Entrez 881405
GenBank AAG08061.1
INSDC AAG08061.1
NCBI Locus Tag PA4674
protein_id(GenBank) gb|AAG08061.1|AE004881_4|gnl|PseudoCAP|PA4674
TIGR NTL03PA04675
UniParc UPI00000C5DBA
UniProtKB Acc Q9HVC1
UniProtKB ID Q9HVC1_PSEAE
UniRef100 UniRef100_Q9HVC1
UniRef50 UniRef50_A6VCC3
UniRef90 UniRef90_A6VCC3

Product

Feature Type CDS
Coding Frame 1
Product Name
Antitoxin HigA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.29
Kyte-Doolittle Hydrophobicity Value -0.163
Molecular Weight (kDa) 11.2
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JPI ANTITOXIN/DNA 03/27/19 Crystal structure of PA4674 in complex with its operator DNA (28bp) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.143 X-RAY DIFFRACTION 100.0
7CSV ANTITOXIN 08/17/20 Pseudomonas aeruginosa antitoxin HigA Pseudomonas aeruginosa PAO1 1.71 X-RAY DIFFRACTION 100.0
7CSW ANTITOXIN/DNA 08/17/20 Pseudomonas aeruginosa antitoxin HigA with pa2440 promoter Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa UCBPP-PA14 1.97 X-RAY DIFFRACTION 100.0
6LB3 ANTITOXIN/DNA 11/13/19 Crystal structure of PA4674 in complex with its operator DNA (18bp) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 2.497 X-RAY DIFFRACTION 100.0
7CSY ANTITOXIN/DNA 08/17/20 Pseudomonas aeruginosa antitoxin HigA with higBA promoter Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa UCBPP-PA14 2.29 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 217 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004324 (343 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4674
Search term: Antitoxin HigA

Human Homologs

References

Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome.
Katz ME, Wright CL, Gartside TS, Cheetham BF, Doidge CV, Moses EK, Rood JI
J Bacteriol 1994 May;176(9):2663-9
PubMed ID: 8169216
The HigB/HigA toxin/antitoxin system of Pseudomonas aeruginosa influences the virulence factors pyochelin, pyocyanin, and biofilm formation.
Wood TL, Wood TK
Microbiologyopen 2016 Jun;5(3):499-511
PubMed ID: 26987441