Pseudomonas aeruginosa PAO1, PA4696 (ilvI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4696
Name
ilvI
Replicon chromosome
Genomic location 5274008 - 5275732 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253384.1
GI 15599890
Affymetrix PA4696_ilvI_at
Entrez 881496
GenBank AAG08082.1
INSDC AAG08082.1
NCBI Locus Tag PA4696
protein_id(GenBank) gb|AAG08082.1|AE004883_6|gnl|PseudoCAP|PA4696
TIGR NTL03PA04697
UniParc UPI00000C5DCC
UniProtKB Acc Q9HVA0
UniProtKB ID Q9HVA0_PSEAE
UniRef100 UniRef100_W0Z0C4
UniRef50 UniRef50_P00893
UniRef90 UniRef90_S6B073

Product

Feature Type CDS
Coding Frame 1
Product Name
acetolactate synthase large subunit
Product Name Confidence: Class 2
Synonyms acetohydroxy-acid synthase III large subunit
AHAS-III, ALS-III
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.99
Kyte-Doolittle Hydrophobicity Value -0.120
Molecular Weight (kDa) 63.0
Isoelectric Point (pI) 6.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 596 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004345 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4696
Search term: ilvI

Human Homologs

References

Unusual organization of the ilvIH promoter of Escherichia coli.
Haughn GW, Squires CH, De Felice M, Largo CT, Calvo JM
J Bacteriol 1985 Jul;163(1):186-98
PubMed ID: 3891724
Isolation and characterization of subunits of acetohydroxy acid synthase isozyme III and reconstitution of the holoenzyme.
Vyazmensky M, Sella C, Barak Z, Chipman DM
Biochemistry 1996 Aug 13;35(32):10339-46
PubMed ID: 8756689
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994