Pseudomonas aeruginosa PAO1, PA4728 (folK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4728
Name
folK
Replicon chromosome
Genomic location 5310726 - 5311214 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253416.1
GI 15599922
Affymetrix PA4728_folK_at
DNASU PaCD00008052
Entrez 881616
GenBank AAG08114.1
INSDC AAG08114.1
NCBI Locus Tag PA4728
protein_id(GenBank) gb|AAG08114.1|AE004886_6|gnl|PseudoCAP|PA4728
TIGR NTL03PA04729
UniParc UPI00000C5DE4
UniProtKB Acc Q9HV71
UniProtKB ID HPPK_PSEAE
UniRef100 UniRef100_Q9HV71
UniRef50 UniRef50_P43777
UniRef90 UniRef90_Q9HV71

Product

Feature Type CDS
Coding Frame 1
Product Name
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Product Name Confidence: Class 2
Synonyms 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.31
Kyte-Doolittle Hydrophobicity Value 0.035
Molecular Weight (kDa) 18.0
Isoelectric Point (pI) 4.56

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 547 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004375 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4728
Search term: folK

Human Homologs

References

Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control.
Liu JD, Parkinson JS
J Bacteriol 1989 Mar;171(3):1254-61
PubMed ID: 2537812
Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.
Talarico TL, Ray PH, Dev IK, Merrill BM, Dallas WS
J Bacteriol 1992 Sep;174(18):5971-7
PubMed ID: 1325970