Pseudomonas aeruginosa PAO1, PA4729 (panB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4729
Name
panB
Replicon chromosome
Genomic location 5311464 - 5312264 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253417.1
GI 15599923
Affymetrix PA4729_panB_at
DNASU PaCD00008053
Entrez 881621
GenBank AAG08115.1
INSDC AAG08115.1
NCBI Locus Tag PA4729
protein_id(GenBank) gb|AAG08115.1|AE004886_7|gnl|PseudoCAP|PA4729
TIGR NTL03PA04730
UniParc UPI0000131262
UniProtKB Acc Q9HV70
UniProtKB ID PANB2_PSEAE
UniRef100 UniRef100_Q9HV70
UniRef50 UniRef50_Q9JZW6
UniRef90 UniRef90_Q9HV70

Product

Feature Type CDS
Coding Frame 1
Product Name
3-methyl-2-oxobutanoate hydroxymethyltransferase
Product Name Confidence: Class 2
Synonyms alpha-ketoisovalerate hydroxymethyltransferase
ketopantoate hydroxymethyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.20
Kyte-Doolittle Hydrophobicity Value 0.271
Molecular Weight (kDa) 27.9
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 533 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004376 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4729
Search term: panB

Human Homologs

References

Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme.
Jones CE, Brook JM, Buck D, Abell C, Smith AG
J Bacteriol 1993 Apr;175(7):2125-30
PubMed ID: 8096212
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994