Pseudomonas aeruginosa PAO1, PA4740 (pnp)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4740
Name
pnp
Replicon chromosome
Genomic location 5323374 - 5325479 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253428.1
GI 15599934
Affymetrix PA4740_pnp_at
Entrez 881671
GenBank AAG08126.1
INSDC AAG08126.1
NCBI Locus Tag PA4740
protein_id(GenBank) gb|AAG08126.1|AE004888_1|gnl|PseudoCAP|PA4740
TIGR NTL03PA04741
UniParc UPI00000C5DEC
UniProtKB Acc Q9HV59
UniProtKB ID PNP_PSEAE
UniRef100 UniRef100_B7V1F2
UniRef50 UniRef50_A6VU33
UniRef90 UniRef90_B7V1F2

Product

Feature Type CDS
Coding Frame 1
Product Name
polyribonucleotide nucleotidyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -20.13
Kyte-Doolittle Hydrophobicity Value -0.070
Molecular Weight (kDa) 75.4
Isoelectric Point (pI) 4.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 663 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004385 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4740
Search term: pnp

Human Homologs

Ensembl 110, assembly GRCh38.p14
polyribonucleotide nucleotidyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:23166]
E-value: 1.0e-114
Percent Identity: 36.8
Ensembl 110, assembly GRCh38.p14
polyribonucleotide nucleotidyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:23166]
E-value: 1.0e-114
Percent Identity: 36.8

References

Promoter activity and transcript mapping in the regulatory region for genes encoding ribosomal protein S15 and polynucleotide phosphorylase of Escherichia coli.
Evans S, Dennis PP
Gene 1985;40(1):15-22
PubMed ID: 3005122
Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1.
Régnier P, Grunberg-Manago M, Portier C
J Biol Chem 1987 Jan 5;262(1):63-8
PubMed ID: 2432069
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994