Pseudomonas aeruginosa PAO1, PA4756 (carB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4756
Name
carB
Replicon chromosome
Genomic location 5339864 - 5343085 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253444.1
GI 15599950
Affymetrix PA4756_carB_at
Entrez 881743
GenBank AAG08142.1
INSDC AAA25764.1
NCBI Locus Tag PA4756
protein_id(GenBank) gb|AAG08142.1|AE004889_5|gnl|PseudoCAP|PA4756
TIGR NTL03PA04757
UniParc UPI0000165ED6
UniProtKB Acc P38100
UniProtKB ID CARB_PSEAE
UniRef100 UniRef100_P38100
UniRef50 UniRef50_P00968
UniRef90 UniRef90_P38100

Product

Feature Type CDS
Coding Frame 1
Product Name
carbamoylphosphate synthetase large subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (117.33 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -33.47
Kyte-Doolittle Hydrophobicity Value -0.096
Molecular Weight (kDa) 117.3
Isoelectric Point (pI) 4.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 624 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004401 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4756
Search term: carB

Human Homologs

Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthase 1 [Source:HGNC Symbol;Acc:HGNC:2323]
E-value: 6.6e-227
Percent Identity: 40.8
Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthase 1 [Source:HGNC Symbol;Acc:HGNC:2323]
E-value: 6.6e-227
Percent Identity: 40.8
Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthase 1 [Source:HGNC Symbol;Acc:HGNC:2323]
E-value: 6.6e-227
Percent Identity: 40.8
Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthase 1 [Source:HGNC Symbol;Acc:HGNC:2323]
E-value: 6.6e-227
Percent Identity: 40.8
Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthase 1 [Source:HGNC Symbol;Acc:HGNC:2323]
E-value: 6.6e-227
Percent Identity: 40.8

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Carbamoylphosphate synthetase from Pseudomonas aeruginosa. Subunit composition, kinetic analysis and regulation.
Abdelal AT, Bussey L, Vickers L
Eur J Biochem 1983 Jan 1;129(3):697-702
PubMed ID: 6402363
Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.
Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT
J Bacteriol 1994 May;176(9):2532-42
PubMed ID: 8169201