Pseudomonas aeruginosa PAO1, PA4854 (purH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4854
Name
purH
Replicon chromosome
Genomic location 5449046 - 5450653 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253541.1
GI 15600047
Affymetrix PA4854_purH_at
Entrez 880046
GenBank AAG08239.1
INSDC AAG08239.1
NCBI Locus Tag PA4854
protein_id(GenBank) gb|AAG08239.1|AE004898_10|gnl|PseudoCAP|PA4854
TIGR NTL03PA04855
UniParc UPI00000C5E3D
UniProtKB Acc Q9HUV9
UniProtKB ID PUR9_PSEAE
UniRef100 UniRef100_B7V1R6
UniRef50 UniRef50_B7LAV3
UniRef90 UniRef90_A6VCW0

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylaminoimidazolecarboxamide formyltransferase
Product Name Confidence: Class 2
Synonyms IMP cyclohydrolase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.48
Kyte-Doolittle Hydrophobicity Value -0.068
Molecular Weight (kDa) 57.7
Isoelectric Point (pI) 6.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4243
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 604 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004483 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4854
Search term: purH

Human Homologs

Ensembl 110, assembly GRCh38.p14
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase [Source:HGNC Symbol;Acc:HGNC:794]
E-value: 7.7e-75
Percent Identity: 34.4

References

Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.
Aiba A, Mizobuchi K
J Biol Chem 1989 Dec 15;264(35):21239-46
PubMed ID: 2687276
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994