Pseudomonas aeruginosa PAO1, PA4855 (purD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4855
Name
purD
Replicon chromosome
Genomic location 5450757 - 5452046 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253542.1
GI 15600048
Affymetrix PA4855_purD_at
Entrez 878236
GenBank AAG08240.1
INSDC AAG08240.1
NCBI Locus Tag PA4855
protein_id(GenBank) gb|AAG08240.1|AE004899_1|gnl|PseudoCAP|PA4855
TIGR NTL03PA04856
UniParc UPI0000132A37
UniProtKB Acc Q9HUV8
UniProtKB ID PUR2_PSEAE
UniRef100 UniRef100_Q9HUV8
UniRef50 UniRef50_P26977
UniRef90 UniRef90_Q9HUV8

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylamine--glycine ligase
Product Name Confidence: Class 2
Synonyms phosphoribosylglycineamide synthetase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.66
Kyte-Doolittle Hydrophobicity Value 0.057
Molecular Weight (kDa) 45.2
Isoelectric Point (pI) 4.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4217
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 627 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004484 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4855
Search term: purD

Human Homologs

Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 3.6e-115
Percent Identity: 52.8
Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 3.6e-115
Percent Identity: 52.8
Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 3.6e-115
Percent Identity: 52.8
Ensembl 110, assembly GRCh38.p14
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:HGNC Symbol;Acc:HGNC:4163]
E-value: 3.6e-115
Percent Identity: 52.8

References

Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization.
Cheng YS, Shen Y, Rudolph J, Stern M, Stubbe J, Flannigan KA, Smith JM
Biochemistry 1990 Jan 9;29(1):218-27
PubMed ID: 2182115
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994