Pseudomonas aeruginosa PAO1, PA4865 (ureA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4865
Name
ureA
Replicon chromosome
Genomic location 5463607 - 5463909 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253552.1
GI 15600058
Affymetrix PA4865_ureA_at
DNASU PaCD00008109
Entrez 882210
GenBank AAG08250.1
INSDC AAG08250.1
NCBI Locus Tag PA4865
protein_id(GenBank) gb|AAG08250.1|AE004900_4|gnl|PseudoCAP|PA4865
TIGR NTL03PA04866
UniParc UPI00000C5E47
UniProtKB Acc Q9HUU8
UniProtKB ID URE3_PSEAE
UniRef100 UniRef100_B7V1S8
UniRef50 UniRef50_B7V1S8
UniRef90 UniRef90_A6VCX2

Product

Feature Type CDS
Coding Frame 1
Product Name
urease gamma subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.53
Kyte-Doolittle Hydrophobicity Value 0.003
Molecular Weight (kDa) 11.0
Isoelectric Point (pI) 5.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-Carboxymethionine DB02899 DrugBank 5.1.4 P41022 Urease subunit gamma 68.0 100 2.7e-29

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 240 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004493 (525 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4865
Search term: ureA

Human Homologs

References

Characterization of a plasmid-encoded urease gene cluster found in members of the family Enterobacteriaceae.
D'Orazio SE, Collins CM
J Bacteriol 1993 Mar;175(6):1860-4
PubMed ID: 8449894