Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4920
|
Name |
nadE
|
Replicon | chromosome |
Genomic location | 5519711 - 5520538 (+ strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_253607.1 |
GI | 15600113 |
Affymetrix | PA4920_nadE_at |
DNASU | PaCD00008129 |
Entrez | 882213 |
GenBank | AAG08305.1 |
INSDC | AAG08305.1 |
NCBI Locus Tag | PA4920 |
protein_id(GenBank) | gb|AAG08305.1|AE004905_3|gnl|PseudoCAP|PA4920 |
TIGR | NTL03PA04921 |
UniParc | UPI000012FCD7 |
UniProtKB Acc | Q9HUP3 |
UniProtKB ID | NADE_PSEAE |
UniRef100 | UniRef100_B7V1Y3 |
UniRef50 | UniRef50_Q8D3T7 |
UniRef90 | UniRef90_A6VD32 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
NH3-dependent NAD synthetase
|
Synonyms | |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -6.37 |
Kyte-Doolittle Hydrophobicity Value | -0.193 |
Molecular Weight (kDa) | 29.7 |
Isoelectric Point (pI) | 5.38 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4XFD | LIGASE | 12/26/14 | Crystal Structure of a NH(3)-dependent NAD(+) synthetase from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.55 | X-RAY DIFFRACTION | 100.0 |
5F23 | LIGASE | 12/01/15 | Crystal structure of NH(3)-dependent NAD(+) synthetase Pseudomonas aeruginosa in complex with NAD | Pseudomonas aeruginosa | 1.5 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004537 (530 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4920
Search term: nadE
Search term: NH3-dependent NAD synthetase
|
The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase.
Willison JC, Tissot G
J Bacteriol 1994 Jun;176(11):3400-2
PubMed ID: 8195100
|
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep 5;277(5331):1453-62
PubMed ID: 9278503
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|