Pseudomonas aeruginosa PAO1, PA4922 (azu)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4922
Name
azu
Replicon chromosome
Genomic location 5521664 - 5522110 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253609.1
GI 15600115
Affymetrix PA4922_azu_at
DNASU PaCD00008130
Entrez 878046
GenBank AAG08307.1
INSDC AAG08307.1
NCBI Locus Tag PA4922
protein_id(GenBank) gb|AAG08307.1|AE004905_5|gnl|PseudoCAP|PA4922
TIGR NTL03PA04923
UniParc UPI00001266B8
UniProtKB Acc P00282
UniProtKB ID AZUR_PSEAE
UniRef100 UniRef100_P00282
UniRef50 UniRef50_P00282
UniRef90 UniRef90_P00282

Product

Feature Type CDS
Coding Frame 1
Product Name
azurin precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.14
Kyte-Doolittle Hydrophobicity Value -0.067
Molecular Weight (kDa) 16.0
Isoelectric Point (pI) 6.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8F5L ELECTRON TRANSPORT 11/14/22 Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110L mutant Pseudomonas aeruginosa 1.15 X-RAY DIFFRACTION 97.3
1JZH ELECTRON TRANSPORT 09/16/01 Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83) Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
8F5K ELECTRON TRANSPORT 11/14/22 Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant Pseudomonas aeruginosa 1.25 X-RAY DIFFRACTION 97.3
4JKN ELECTRON TRANSPORT 03/10/13 Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A Pseudomonas aeruginosa 1.536 X-RAY DIFFRACTION 100.0
1VLX ELECTRON TRANSPORT 10/08/96 STRUCTURE OF ELECTRON TRANSFER (COBALT-PROTEIN) Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
4HZ1 ELECTRON TRANSPORT 11/14/12 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 100.0
4AZU ELECTRON TRANSPORT(COPPER BINDING) 06/23/93 CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
5AZU ELECTRON TRANSPORT(COPPER BINDING) 06/23/93 CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
1E65 ELECTRON TRANSPORT 08/08/00 Azurin from Pseudomonas aeruginosa, apo form PSEUDOMONAS AERUGINOSA 1.85 X-RAY DIFFRACTION 100.0
1E67 ELECTRON TRANSPORT 08/09/00 Zn-Azurin from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.14 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Tris-Hydroxymethyl-Methyl-Ammonium DB03570 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
Tetra(Imidazole)Diaquacopper (Ii) DB03840 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
(1,10 Phenanthroline)-(Tri-Carbon Monoxide) Rhenium (I) DB04100 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
S-Hydroxycysteine DB01915 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
4,7-Dimethyl-[1,10]Phenanthroline DB02586 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
1,1'-HEXANE-1,6-DIYLBIS(1H-IMIDAZOLE) DB06968 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
Tetra(Imidazole)Diaquacopper (I) DB04231 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
lambda-bis(2,2'-bipyridine)imidazole ruthenium (II) DB03871 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
lambda-bis(2,2'-bipyridine)imidazole osmium (II) DB03492 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78
1-[Pyrrol-1-Yl-2,5-Dione-Methoxymethyl]-Pyrrole-2,5-Dione DB04085 DrugBank 5.1.4 P00282 Azurin 100.0 148 1.2e-78

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004539 (509 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4922
Search term: azu
Search term: azurin precursor

Human Homologs

References

Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Type 1, blue copper proteins constitute a respiratory nitrite-reducing system in Pseudomonas aureofaciens.
Zumft WG, Gotzmann DJ, Kroneck PM
Eur J Biochem 1987 Oct 15;168(2):301-7
PubMed ID: 3665926
The azurin gene from Pseudomonas aeruginosa. Cloning and characterization.
Arvidsson RH, Nordling M, Lundberg LG
Eur J Biochem 1989 Jan 15;179(1):195-200
PubMed ID: 2537198
Complex formation between the copper protein, azurin and the cytochrome c peroxidase of Pseudomonas aeruginosa.
Brittain T, Greenwood C
J Inorg Biochem 1992 Oct 1;48(1):71-7
PubMed ID: 1326600
Crystal structures of modified apo-His117Gly and apo-His46Gly mutants of Pseudomonas aeruginosa azurin.
Hammann C, van Pouderoyen G, Nar H, Gomis Rüth FX, Messerschmidt A, Huber R, den Blaauwen T, Canters GW
J Mol Biol 1997 Feb 21;266(2):357-66
PubMed ID: 9047369
Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5.
Matsushita K, Yamashita T, Aoki N, Toyama H, Adachi O
Biochemistry 1999 May 11;38(19):6111-8
PubMed ID: 10320337
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142