Pseudomonas aeruginosa PAO1, PA4936

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4936
Name
Synonym: spoU
Synonym: yjfH
Replicon chromosome
Genomic location 5537953 - 5538699 (- strand)
Transposon Mutants 10 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253623.1
GI 15600129
Affymetrix PA4936_at
Entrez 877647
GenBank AAG08321.1
INSDC AAG08321.1
NCBI Locus Tag PA4936
protein_id(GenBank) gb|AAG08321.1|AE004906_9|gnl|PseudoCAP|PA4936
TIGR NTL03PA04937
UniParc UPI00000C5E85
UniProtKB Acc Q9HUM8
UniProtKB ID RLMB_PSEAE
UniRef100 UniRef100_Q9HUM8
UniRef50 UniRef50_Q8ZIV4
UniRef90 UniRef90_Q9HUM8

Product

Feature Type CDS
Coding Frame 1
Product Name
probable rRNA methylase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.56
Kyte-Doolittle Hydrophobicity Value -0.053
Molecular Weight (kDa) 27.1
Isoelectric Point (pI) 7.18

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004551 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4936

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial rRNA methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:26202]
E-value: 5.1e-21
Percent Identity: 32.1
Ensembl 110, assembly GRCh38.p14
mitochondrial rRNA methyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:26202]
E-value: 5.1e-21
Percent Identity: 32.1

References

The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.
Persson BC, Jäger G, Gustafsson C
Nucleic Acids Res 1997 Oct 15;25(20):4093-7
PubMed ID: 9321663