Pseudomonas aeruginosa PAO1, PA4945 (miaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4945
Name
miaA
Replicon chromosome
Genomic location 5548751 - 5549722 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253632.1
GI 15600138
Affymetrix PA4945_miaA_at
DNASU PaCD00008140
Entrez 878016
GenBank AAG08330.1
INSDC AAG08330.1
NCBI Locus Tag PA4945
protein_id(GenBank) gb|AAG08330.1|AE004907_8|gnl|PseudoCAP|PA4945
TIGR NTL03PA04946
UniParc UPI000012F0C8
UniProtKB Acc Q9HUL9
UniProtKB ID MIAA_PSEAE
UniRef100 UniRef100_B7V208
UniRef50 UniRef50_A4VQP1
UniRef90 UniRef90_A6VD58

Product

Feature Type CDS
Coding Frame 1
Product Name
delta 2-isopentenylpyrophosphate transferase
Product Name Confidence: Class 2
Synonyms tRNA prenyltransferase
tRNA:isopentenyladenine transferase
Evidence for Translation
Charge (pH 7) 1.90
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 35.8
Isoelectric Point (pI) 7.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3CRQ TRANSFERASE 04/07/08 Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 99.7
3CRM TRANSFERASE 04/07/08 Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 99.7
3CRR TRANSFERASE 04/07/08 Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 584 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004560 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4945
Search term: miaA

Human Homologs

References

Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations.
Connolly DM, Winkler ME
J Bacteriol 1991 Mar;173(5):1711-21
PubMed ID: 1999389
Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium.
Tsui HT, Mandavilli BS, Winkler ME
Nucleic Acids Res 1992 May 11;20(9):2379
PubMed ID: 1594459
Regulation of substrate recognition by the MiaA tRNA prenyltransferase modification enzyme of Escherichia coli K-12.
Leung HC, Chen Y, Winkler ME
J Biol Chem 1997 May 16;272(20):13073-83
PubMed ID: 9148919