Pseudomonas aeruginosa PAO1, PA4959 (fimX)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4959
Name	fimX Synonym: fimX
Replicon	chromosome
Genomic location	5565493 - 5567568 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	3 transposon mutants in orthologs
Comment	FimX exhibits phosphodiesterase, but not diguanylate cyclase, activity in vitro.

Cross-References

RefSeq	NP_253646.1
GI	15600152
Affymetrix	PA4959_at
Entrez	878416
GenBank	AAG08344.1
INSDC	AAG08344.1
NCBI Locus Tag	PA4959
protein_id(GenBank)	gb|AAG08344.1|AE004908_10|gnl|PseudoCAP|PA4959
TIGR	NTL03PA04960
UniParc	UPI00000C5E96
UniProtKB Acc	Q9HUK6
UniProtKB ID	Q9HUK6_PSEAE
UniRef100	UniRef100_Q9HUK6
UniRef50	UniRef50_Q9HUK6
UniRef90	UniRef90_Q9HUK6

Product

Feature Type	CDS
Coding Frame	1
Product Name	FimX Product Name Confidence: Class 1
Synonyms	FimX .New product name confidence: Class 1 (protein function experimentally demonstrated in P
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-24.10
Kyte-Doolittle Hydrophobicity Value	-0.195
Molecular Weight (kDa)	76.0
Isoelectric Point (pI)	4.88

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3HV9	HYDROLASE	06/15/09	Crystal structure of FimX EAL domain from Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.298	X-RAY DIFFRACTION	100.0
4J40	HYDROLASE	02/06/13	Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.99	X-RAY DIFFRACTION	100.0
3HVA	TRANSFERASE	06/15/09	Crystal structure of FimX GGDEF domain from Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.042	X-RAY DIFFRACTION	100.0
4AFY	HYDROLASE	01/23/12	Crystal structure of the FimX EAL domain in complex with reaction product pGpG	PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT	2.01	X-RAY DIFFRACTION	100.0
3HV8	HYDROLASE	06/15/09	Crystal structure of FimX EAL domain from Pseudomonas aeruginosa bound to c-di-GMP	Pseudomonas aeruginosa PAO1	1.445	X-RAY DIFFRACTION	100.0
4AG0	HYDROLASE	01/23/12	Crystal structure of FimX EAL domain	PSEUDOMONAS AERUGINOSA	2.304	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 436 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004574 (353 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4959 Search term: fimX Search term: FimX

Human Homologs

References

FimX, a multidomain protein connecting environmental signals to twitching motility in Pseudomonas aeruginosa. Huang B, Whitchurch CB, Mattick JS J Bacteriol 2003 Dec;185(24):7068-76 PubMed ID: 14645265

Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa. Kazmierczak BI, Lebron MB, Murray TS Mol Microbiol 2006 May;60(4):1026-43 PubMed ID: 16677312