Pseudomonas aeruginosa PAO1, PA5036 (gltB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5036
Name
gltB
Synonym: aspB
Replicon chromosome
Genomic location 5667696 - 5672141 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253723.1
GI 15600229
Affymetrix PA5036_gltB_at
Entrez 881222
GenBank AAG08421.1
INSDC AAG08421.1
NCBI Locus Tag PA5036
protein_id(GenBank) gb|AAG08421.1|AE004916_10|gnl|PseudoCAP|PA5036
TIGR NTL03PA05037
UniParc UPI00000C5ED9
UniProtKB Acc Q9HUD5
UniProtKB ID Q9HUD5_PSEAE
UniRef100 UniRef100_Q9HUD5
UniRef50 UniRef50_P09831
UniRef90 UniRef90_M4X496

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamate synthase large chain precursor
Product Name Confidence: Class 2
Synonyms GltS alpha chain
NADPH-GOGAT
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.30
Kyte-Doolittle Hydrophobicity Value -0.143
Molecular Weight (kDa) 161.6
Isoelectric Point (pI) 6.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 544 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004641 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5036
Search term: gltB

Human Homologs

References

Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12.
Oliver G, Gosset G, Sanchez-Pescador R, Lozoya E, Ku LM, Flores N, Becerril B, Valle F, Bolivar F
Gene 1987;60(1):1-11
PubMed ID: 3326786
Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12.
Gosset G, Merino E, Recillas F, Oliver G, Becerril B, Bolivar F
Protein Seq Data Anal 1989;2(1):9-16
PubMed ID: 2643092
Glucose-Binding of Periplasmic Protein GltB Activates GtrS-GltR Two-Component System in Pseudomonas aeruginosa.
Xu C, Cao Q, Lan L
Microorganisms 2021 Feb 21;9(2)
PubMed ID: 33670077