Pseudomonas aeruginosa PAO1, PA5039 (aroK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5039
Name
aroK
Replicon chromosome
Genomic location 5675184 - 5675702 (- strand)

Cross-References

RefSeq NP_253726.1
GI 15600232
Affymetrix PA5039_aroK_at
Entrez 880961
GenBank AAG08424.1
INSDC AAA16705.1
NCBI Locus Tag PA5039
protein_id(GenBank) gb|AAG08424.1|AE004917_3|gnl|PseudoCAP|PA5039
TIGR NTL03PA05040
UniParc UPI000012602A
UniProtKB Acc P34003
UniProtKB ID AROK_PSEAE
UniRef100 UniRef100_B7V3D2
UniRef50 UniRef50_B7V3D2
UniRef90 UniRef90_B7V3D2

Product

Feature Type CDS
Coding Frame 1
Product Name
shikimate kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.66
Kyte-Doolittle Hydrophobicity Value -0.246
Molecular Weight (kDa) 19.2
Isoelectric Point (pI) 8.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 563 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004644 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5039
Search term: aroK
Search term: shikimate kinase

Human Homologs

Ensembl 110, assembly GRCh38.p14
threonine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26160]
E-value: 2.7e-05
Percent Identity: 26.0
Ensembl 110, assembly GRCh38.p14
threonine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26160]
E-value: 2.7e-05
Percent Identity: 26.0

References

Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.
Ely B, Pittard J
J Bacteriol 1979 Jun;138(3):933-43
PubMed ID: 222728
The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product.
Millar G, Lewendon A, Hunter MG, Coggins JR
Biochem J 1986 Jul 15;237(2):427-37
PubMed ID: 3026317
Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli.
Løbner-Olesen A, Marinus MG
J Bacteriol 1992 Jan;174(2):525-9
PubMed ID: 1309529
A reassessment of the relationship between aroK- and aroL-encoded shikimate kinase enzymes of Escherichia coli.
Whipp MJ, Pittard AJ
J Bacteriol 1995 Mar;177(6):1627-9
PubMed ID: 7883721