Pseudomonas aeruginosa PAO1, PA5045 (ponA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA5045
|
| Name |
ponA
Synonym: mrcA |
| Replicon | chromosome |
| Genomic location | 5680898 - 5683366 (+ strand) |
| Transposon Mutants | 4 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_253732.1 |
| GI | 15600238 |
| Affymetrix | PA5045_ponA_at |
| Entrez | 881163 |
| GenBank | AAG08430.1 |
| INSDC | AAC36826.1 |
| NCBI Locus Tag | PA5045 |
| protein_id(GenBank) | gb|AAG08430.1|AE004917_9|gnl|PseudoCAP|PA5045 |
| TIGR | NTL03PA05046 |
| UniParc | UPI000013139F |
| UniProtKB Acc | Q07806 |
| UniProtKB ID | PBPA_PSEAE |
| UniRef100 | UniRef100_Q07806 |
| UniRef50 | UniRef50_Q07806 |
| UniRef90 | UniRef90_Q07806 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
penicillin-binding protein 1A
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (91.20 kDa, PMID:21751344)
|
| Charge (pH 7) | -5.09 |
| Kyte-Doolittle Hydrophobicity Value | -0.339 |
| Molecular Weight (kDa) | 91.2 |
| Isoelectric Point (pI) | 6.01 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 4OON | transferase/transferase inhibitor | 02/03/14 | Crystal structure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) | Pseudomonas aeruginosa PAO1 | 3.2 | X-RAY DIFFRACTION | 100.0 |
Drugs Targeting this Protein
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
| Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
| Cefpiramide | DB00430 | DrugBank | 5.1.4 | Q07806 | Penicillin-binding protein 1A | 100.0 | 822 | 0.0e+00 |
| Cefoperazone | DB01329 | DrugBank | 5.1.4 | Q07806 | Penicillin-binding protein 1A | 100.0 | 822 | 0.0e+00 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 555 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG004648 (535 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA5045
Search term: ponA
Search term: penicillin-binding protein 1A
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12.
Broome-Smith JK, Edelman A, Yousif S, Spratt BG
Eur J Biochem 1985 Mar 1;147(2):437-46
PubMed ID: 3882429
|
|
Sequence of the ponA gene and characterization of the penicillin-binding protein 1A of Pseudomonas aeruginosa PAO1.
Handfield J, Gagnon L, Dargis M, Huletsky A
Gene 1997 Oct 15;199(1-2):49-56
PubMed ID: 9358038
|
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|