Pseudomonas aeruginosa PAO1, PA5046

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5046
Name
Replicon chromosome
Genomic location 5683471 - 5684739 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253733.1
GI 15600239
Affymetrix PA5046_at
Entrez 881142
GenBank AAG08431.1
INSDC AAG08431.1
NCBI Locus Tag PA5046
protein_id(GenBank) gb|AAG08431.1|AE004918_1|gnl|PseudoCAP|PA5046
TIGR NTL03PA05047
UniParc UPI00000C5EDB
UniProtKB Acc Q9HUD3
UniProtKB ID Q9HUD3_PSEAE
UniRef100 UniRef100_Q9HUD3
UniRef50 UniRef50_O34962
UniRef90 UniRef90_Q4KJJ6

Product

Feature Type CDS
Coding Frame 1
Product Name
malic enzyme
Product Name Confidence: Class 2
Synonyms pyruvic-malic carboxylase
malate dehydrogenase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.96
Kyte-Doolittle Hydrophobicity Value 0.001
Molecular Weight (kDa) 45.4
Isoelectric Point (pI) 4.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 551 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004649 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5046
Search term: malic enzyme

Human Homologs

References

Structure and properties of malic enzyme from Bacillus stearothermophilus.
Kobayashi K, Doi S, Negoro S, Urabe I, Okada H
J Biol Chem 1989 Feb 25;264(6):3200-5
PubMed ID: 2644282
NAD(+)-dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation.
Driscoll BT, Finan TM
Mol Microbiol 1993 Mar;7(6):865-73
PubMed ID: 8387144
NADP+ -dependent malic enzyme of Rhizobium meliloti.
Driscoll BT, Finan TM
J Bacteriol 1996 Apr;178(8):2224-31
PubMed ID: 8636022
Properties of NAD(+)- and NADP(+)-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti and differential expression of their genes in nitrogen-fixing bacteroids.
Driscoll BT, Finan TM
Microbiology (Reading) 1997 Feb;143 ( Pt 2):489-498
PubMed ID: 9043124
Chimeric structure of the NAD(P)+- and NADP+-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti.
Mitsch MJ, Voegele RT, Cowie A, Osteras M, Finan TM
J Biol Chem 1998 Apr 10;273(15):9330-6
PubMed ID: 9535928
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994