Pseudomonas aeruginosa PAO1, PA5051 (argS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5051
Name
argS
Replicon chromosome
Genomic location 5689964 - 5691727 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253738.1
GI 15600244
Affymetrix PA5051_argS_at
Entrez 881194
GenBank AAG08436.1
INSDC AAG08436.1
NCBI Locus Tag PA5051
protein_id(GenBank) gb|AAG08436.1|AE004918_6|gnl|PseudoCAP|PA5051
TIGR NTL03PA05052
UniParc UPI0000136658
UniProtKB Acc Q9HUC8
UniProtKB ID SYR_PSEAE
UniRef100 UniRef100_Q9HUC8
UniRef50 UniRef50_Q9KQC6
UniRef90 UniRef90_Q9HUC8

Product

Feature Type CDS
Coding Frame 1
Product Name
arginyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms arginine--tRNA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.53
Kyte-Doolittle Hydrophobicity Value -0.187
Molecular Weight (kDa) 65.2
Isoelectric Point (pI) 5.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004653 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5051
Search term: argS

Human Homologs

Ensembl 110, assembly GRCh38.p14
arginyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:9870]
E-value: 8.4e-99
Percent Identity: 37.0

References

Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.
Eriani G, Dirheimer G, Gangloff J
Nucleic Acids Res 1989 Jul 25;17(14):5725-36
PubMed ID: 2668891
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T
DNA Res 1996 Dec 31;3(6):379-92
PubMed ID: 9097040
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994