Pseudomonas aeruginosa PAO1, PA5066 (hisI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5066
Name
hisI
Replicon chromosome
Genomic location 5705794 - 5706198 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253753.1
GI 15600259
Affymetrix PA5066_hisI_at
DNASU PaCD00008178
Entrez 878019
GenBank AAG08451.1
INSDC AAG08451.1
NCBI Locus Tag PA5066
protein_id(GenBank) gb|AAG08451.1|AE004920_1|gnl|PseudoCAP|PA5066
TIGR NTL03PA05067
UniParc UPI000012C7AE
UniProtKB Acc Q9HUB7
UniProtKB ID HIS3_PSEAE
UniRef100 UniRef100_B7V3F9
UniRef50 UniRef50_A1WFT0
UniRef90 UniRef90_B7V3F9

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosyl-AMP cyclohydrolase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.93
Kyte-Doolittle Hydrophobicity Value -0.465
Molecular Weight (kDa) 15.4
Isoelectric Point (pI) 6.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 579 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004667 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5066
Search term: hisI

Human Homologs

References

Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons.
Carlomagno MS, Chiariotti L, Alifano P, Nappo AG, Bruni CB
J Mol Biol 1988 Oct 5;203(3):585-606
PubMed ID: 3062174
Cloning of the Rhodobacter sphaeroides hisL gene: unifunctionality of the encoded protein and lack of linkage to other his genes.
Oriol E, Méndez-Alvarez S, Barbé J, Gibert I
Microbiology (Reading) 1996 Aug;142 ( Pt 8):2071-8
PubMed ID: 8760919