Pseudomonas aeruginosa PAO1, PA5078 (opgG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5078
Name
opgG
Synonym: mdoG
Replicon chromosome
Genomic location 5716848 - 5718425 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253765.1
GI 15600271
Affymetrix PA5078_at
Entrez 879629
GenBank AAG08463.1
INSDC AAG08463.1
NCBI Locus Tag PA5078
protein_id(GenBank) gb|AAG08463.1|AE004920_13|gnl|PseudoCAP|PA5078
TIGR NTL03PA05079
UniParc UPI00000C5EED
UniProtKB Acc Q9HUA5
UniProtKB ID OPGG_PSEAE
UniRef100 UniRef100_B7V3H1
UniRef50 UniRef50_Q9F496
UniRef90 UniRef90_A6VDK1

Product

Feature Type CDS
Coding Frame 1
Product Name
OpgG
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.05
Kyte-Doolittle Hydrophobicity Value -0.491
Molecular Weight (kDa) 59.4
Isoelectric Point (pI) 6.98

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4232
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 111 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004679 (524 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5078
Search term: opgG
Search term: OpgG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular analysis of a pathogenicity locus in Pseudomonas syringae pv. syringae.
Mukhopadhyay P, Williams J, Mills D
J Bacteriol 1988 Dec;170(12):5479-88
PubMed ID: 3192509
Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae.
Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP
Mol Microbiol 1993 Oct;10(2):329-40
PubMed ID: 7934824
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Linear osmoregulated periplasmic glucans are encoded by the opgGH locus of Pseudomonas aeruginosa.
Lequette Y, Rollet E, Delangle A, Greenberg EP, Bohin JP
Microbiology (Reading) 2007 Oct;153(Pt 10):3255-3263
PubMed ID: 17906125