Pseudomonas aeruginosa PAO1, PA5163 (rmlA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5163
Name
rmlA
Synonym: rfbA
Replicon chromosome
Genomic location 5812241 - 5813122 (+ strand)

Cross-References

RefSeq NP_253850.1
GI 15600356
Affymetrix PA5163_rmlA_at
Entrez 879990
GenBank AAG08548.1
INSDC AAG08548.1
NCBI Locus Tag PA5163
protein_id(GenBank) gb|AAG08548.1|AE004929_3|gnl|PseudoCAP|PA5163
TIGR NTL03PA05164
UniParc UPI0000053FB9
UniProtKB Acc Q9HU22
UniProtKB ID Q9HU22_PSEAE
UniRef100 UniRef100_Q9HU22
UniRef50 UniRef50_P37744
UniRef90 UniRef90_Q9HU22

Product

Feature Type CDS
Coding Frame 1
Product Name
glucose-1-phosphate thymidylyltransferase
Product Name Confidence: Class 2
Synonyms dTDP-glucose pyrophosphorylase
dTDP-glucose synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.83
Kyte-Doolittle Hydrophobicity Value -0.194
Molecular Weight (kDa) 32.5
Isoelectric Point (pI) 5.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ASJ TRANSFERASE 05/01/12 Pseudomonas aeruginosa RmlA in complex with allosteric inhibitor PSEUDOMONAS AERUGINOSA 2.25 X-RAY DIFFRACTION 100.0
4ASY TRANSFERASE 05/03/12 Pseudomonas aeruginosa RmlA in complex with allosteric inhibitor PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
1G23 TRANSFERASE 10/16/00 THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). GLUCOSE-1-PHOSPHATE COMPLEX. Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0
1G1L TRANSFERASE 10/12/00 THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TDP-GLUCOSE COMPLEX. Pseudomonas aeruginosa 1.77 X-RAY DIFFRACTION 100.0
1FXO TRANSFERASE 09/26/00 THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TMP COMPLEX. Pseudomonas aeruginosa 1.66 X-RAY DIFFRACTION 100.0
1G2V TRANSFERASE 10/21/00 THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TTP COMPLEX. Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Thymidine-5'-Triphosphate DB02452 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
2'-Deoxy-Thymidine-Beta-L-Rhamnose DB03723 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
Thymidine DB04485 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
alpha-D-glucose-1-phosphate DB02843 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
Citric acid DB04272 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
2'deoxy-Thymidine-5'-Diphospho-Alpha-D-Glucose DB03751 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168
Thymidine monophosphate DB01643 DrugBank 5.1.4 Q9HU22 Glucose-1-phosphate thymidylyltransferase 100.0 293 3.3e-168

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 636 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004754 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5163
Search term: rmlA

Human Homologs

Ensembl 110, assembly GRCh38.p14
eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259]
E-value: 3.2e-06
Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14
eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259]
E-value: 3.2e-06
Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14
eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259]
E-value: 3.2e-06
Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14
eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259]
E-value: 3.2e-06
Percent Identity: 39.3

References

Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster.
Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P
J Bacteriol 1994 Jul;176(13):4144-56
PubMed ID: 7517391
Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene.
Marolda CL, Valvano MA
J Bacteriol 1995 Oct;177(19):5539-46
PubMed ID: 7559340