Pseudomonas aeruginosa PAO1, PA5171 (arcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5171
Name
arcA
Replicon chromosome
Genomic location 5822380 - 5823636 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_253858.1
GI 15600364
Affymetrix PA5171_arcA_at
DNASU PaCD00008223
Entrez 881801
GenBank AAG08556.1
INSDC -
NCBI Locus Tag PA5171
protein_id(GenBank) gb|AAG08556.1|AE004930_2|gnl|PseudoCAP|PA5171
TIGR NTL03PA05172
UniParc UPI0000033BA6
UniProtKB Acc P13981
UniProtKB ID ARCA_PSEAE
UniRef100 UniRef100_P13981
UniRef50 UniRef50_P13981
UniRef90 UniRef90_P13981

Product

Feature Type CDS
Coding Frame 1
Product Name
arginine deiminase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (46.44 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.03
Kyte-Doolittle Hydrophobicity Value -0.219
Molecular Weight (kDa) 46.4
Isoelectric Point (pI) 5.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2A9G HYDROLASE 07/11/05 Structure of C406A arginine deiminase in complex with L-arginine Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.8
1RXX HYDROLASE 12/18/03 Structure of arginine deiminase Pseudomonas aeruginosa 2.45 X-RAY DIFFRACTION 100.0
2ACI HYDROLASE 07/18/05 Structure of D166A arginine deiminase Pseudomonas aeruginosa 2.5 X-RAY DIFFRACTION 99.8
2ABR HYDROLASE 07/15/05 Structure of D280A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate Pseudomonas aeruginosa 2.9 X-RAY DIFFRACTION 99.8
2AAF HYDROLASE 07/13/05 Structure of H278A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 99.8

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
L-CYSTEIN-S-1-(IMINOMETHYL)-L-ORNITHINE DB04671 DrugBank 5.1.4 P13981 Arginine deiminase 100.0 418 1.8e-247

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 161 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004759 (487 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5171
Search term: arcA

Human Homologs

References

The arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa contains an additional gene, arcD, encoding a membrane protein.
Lüthi E, Baur H, Gamper M, Brunner F, Villeval D, Mercenier A, Haas D
Gene 1990 Mar 1;87(1):37-43
PubMed ID: 2158926
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878