Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5171
|
Name |
arcA
|
Replicon | chromosome |
Genomic location | 5822380 - 5823636 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_253858.1 |
GI | 15600364 |
Affymetrix | PA5171_arcA_at |
DNASU | PaCD00008223 |
Entrez | 881801 |
GenBank | AAG08556.1 |
INSDC | - |
NCBI Locus Tag | PA5171 |
protein_id(GenBank) | gb|AAG08556.1|AE004930_2|gnl|PseudoCAP|PA5171 |
TIGR | NTL03PA05172 |
UniParc | UPI0000033BA6 |
UniProtKB Acc | P13981 |
UniProtKB ID | ARCA_PSEAE |
UniRef100 | UniRef100_P13981 |
UniRef50 | UniRef50_P13981 |
UniRef90 | UniRef90_P13981 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
arginine deiminase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (46.44 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -8.03 |
Kyte-Doolittle Hydrophobicity Value | -0.219 |
Molecular Weight (kDa) | 46.4 |
Isoelectric Point (pI) | 5.50 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2A9G | HYDROLASE | 07/11/05 | Structure of C406A arginine deiminase in complex with L-arginine | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 99.8 |
1RXX | HYDROLASE | 12/18/03 | Structure of arginine deiminase | Pseudomonas aeruginosa | 2.45 | X-RAY DIFFRACTION | 100.0 |
2ACI | HYDROLASE | 07/18/05 | Structure of D166A arginine deiminase | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 99.8 |
2ABR | HYDROLASE | 07/15/05 | Structure of D280A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 99.8 |
2AAF | HYDROLASE | 07/13/05 | Structure of H278A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 99.8 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
L-CYSTEIN-S-1-(IMINOMETHYL)-L-ORNITHINE | DB04671 | DrugBank | 5.1.4 | P13981 | Arginine deiminase | 100.0 | 418 | 1.8e-247 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 161 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004759 (487 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5171
Search term: arcA
Search term: arginine deiminase
|
The arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa contains an additional gene, arcD, encoding a membrane protein.
Lüthi E, Baur H, Gamper M, Brunner F, Villeval D, Mercenier A, Haas D
Gene 1990 Mar 1;87(1):37-43
PubMed ID: 2158926
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|