Pseudomonas aeruginosa PAO1, PA5175 (cysQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5175
Name
cysQ
Replicon chromosome
Genomic location 5828086 - 5828907 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentBLAST: PA5175 identified by strong homology (E = 10^-113) with Pseudomonas mendocina ymp 3(2),5-biphosphate nucleotidase. Modeling evidence for this reaction.

Cross-References

RefSeq NP_253862.1
GI 15600368
Affymetrix PA5175_cysQ_at
DNASU PaCD00008225
Entrez 881784
GenBank AAG08560.1
INSDC AAG08560.1
NCBI Locus Tag PA5175
protein_id(GenBank) gb|AAG08560.1|AE004930_6|gnl|PseudoCAP|PA5175
TIGR NTL03PA05176
UniParc UPI00000C5F38
UniProtKB Acc Q9HU14
UniProtKB ID Q9HU14_PSEAE
UniRef100 UniRef100_Q9HU14
UniRef50 UniRef50_Q4KJY9
UniRef90 UniRef90_A6VDU7

Product

Feature Type CDS
Coding Frame 1
Product Name
3,5-bisphosphate nucleotidase CysQ
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.43
Kyte-Doolittle Hydrophobicity Value -0.144
Molecular Weight (kDa) 29.8
Isoelectric Point (pI) 4.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 513 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004763 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5175
Search term: cysQ

Human Homologs

References

cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth.
Neuwald AF, Krishnan BR, Brikun I, Kulakauskas S, Suziedelis K, Tomcsanyi T, Leyh TS, Berg DE
J Bacteriol 1992 Jan;174(2):415-25
PubMed ID: 1729235
A rice HAL2-like gene encodes a Ca(2+)-sensitive 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase and complements yeast met22 and Escherichia coli cysQ mutations.
Peng Z, Verma DP
J Biol Chem 1995 Dec 8;270(49):29105-10
PubMed ID: 7493934
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P, Valvano MA, Messner P
J Bacteriol 2002 Jan;184(2):363-9
PubMed ID: 11751812