Pseudomonas aeruginosa PAO1, PA5185

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5185
Name
Replicon chromosome
Genomic location 5837449 - 5837892 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentPA5185 showed significant thioesterase activity against propionyl-CoA

Cross-References

RefSeq NP_253872.1
GI 15600378
Affymetrix PA5185_at
DNASU PaCD00008231
Entrez 881521
GenBank AAG08570.1
INSDC AAG08570.1
NCBI Locus Tag PA5185
protein_id(GenBank) gb|AAG08570.1|AE004931_7|gnl|PseudoCAP|PA5185
TIGR NTL03PA05186
UniParc UPI00000C5F41
UniProtKB Acc Q9HU04
UniProtKB ID Q9HU04_PSEAE
UniRef100 UniRef100_Q9HU04
UniRef50 UniRef50_Q0KBV1
UniRef90 UniRef90_Q9HU04

Product

Feature Type CDS
Coding Frame 1
Product Name
thiolester hydrolase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.11
Kyte-Doolittle Hydrophobicity Value -0.095
Molecular Weight (kDa) 16.5
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2O5U HYDROLASE 12/06/06 Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (C222). Pseudomonas aeruginosa 1.91 X-RAY DIFFRACTION 100.0
2O6U HYDROLASE 12/08/06 Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- new crystal form. Pseudomonas aeruginosa 3.01 X-RAY DIFFRACTION 100.0
2AV9 HYDROLASE 08/29/05 Crystal Structure of the PA5185 protein from Pseudomonas Aeruginosa Strain PAO1. Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 100.0
2O6B HYDROLASE 12/07/06 Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- new crystal form. Pseudomonas aeruginosa 3.21 X-RAY DIFFRACTION 100.0
2O6T HYDROLASE 12/08/06 Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (P2221). Pseudomonas aeruginosa 2.55 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 190 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004772 (470 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5185

Human Homologs

References

Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1.
Chruszcz M, Zimmerman MD, Wang S, Koclega KD, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W
Cryst Growth Des 2008 Nov 5;8(11):4054-4061
PubMed ID: 19898606
The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases.
Dillon SC, Bateman A
BMC Bioinformatics 2004 Aug 12;5:109
PubMed ID: 15307895
Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801.
Gonzalez CF, Tchigvintsev A, Brown G, Flick R, Evdokimova E, Xu X, Osipiuk J, Cuff ME, Lynch S, Joachimiak A, Savchenko A, Yakunin AF
Biochem J 2012 Jun 15;444(3):445-55
PubMed ID: 22439787