Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5185
|
Name |
|
Replicon | chromosome |
Genomic location | 5837449 - 5837892 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | PA5185 showed significant thioesterase activity against propionyl-CoA |
RefSeq | NP_253872.1 |
GI | 15600378 |
Affymetrix | PA5185_at |
DNASU | PaCD00008231 |
Entrez | 881521 |
GenBank | AAG08570.1 |
INSDC | AAG08570.1 |
NCBI Locus Tag | PA5185 |
protein_id(GenBank) | gb|AAG08570.1|AE004931_7|gnl|PseudoCAP|PA5185 |
TIGR | NTL03PA05186 |
UniParc | UPI00000C5F41 |
UniProtKB Acc | Q9HU04 |
UniProtKB ID | Q9HU04_PSEAE |
UniRef100 | UniRef100_Q9HU04 |
UniRef50 | UniRef50_Q0KBV1 |
UniRef90 | UniRef90_Q9HU04 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
thiolester hydrolase
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -1.11 |
Kyte-Doolittle Hydrophobicity Value | -0.095 |
Molecular Weight (kDa) | 16.5 |
Isoelectric Point (pI) | 6.51 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2O5U | HYDROLASE | 12/06/06 | Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (C222). | Pseudomonas aeruginosa | 1.91 | X-RAY DIFFRACTION | 100.0 |
2O6U | HYDROLASE | 12/08/06 | Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- new crystal form. | Pseudomonas aeruginosa | 3.01 | X-RAY DIFFRACTION | 100.0 |
2AV9 | HYDROLASE | 08/29/05 | Crystal Structure of the PA5185 protein from Pseudomonas Aeruginosa Strain PAO1. | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 100.0 |
2O6B | HYDROLASE | 12/07/06 | Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- new crystal form. | Pseudomonas aeruginosa | 3.21 | X-RAY DIFFRACTION | 100.0 |
2O6T | HYDROLASE | 12/08/06 | Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (P2221). | Pseudomonas aeruginosa | 2.55 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 190 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004772 (470 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5185
Search term: thiolester hydrolase
|
Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1.
Chruszcz M, Zimmerman MD, Wang S, Koclega KD, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W
Cryst Growth Des 2008 Nov 5;8(11):4054-4061
PubMed ID: 19898606
|
The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases.
Dillon SC, Bateman A
BMC Bioinformatics 2004 Aug 12;5:109
PubMed ID: 15307895
|
Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801.
Gonzalez CF, Tchigvintsev A, Brown G, Flick R, Evdokimova E, Xu X, Osipiuk J, Cuff ME, Lynch S, Joachimiak A, Savchenko A, Yakunin AF
Biochem J 2012 Jun 15;444(3):445-55
PubMed ID: 22439787
|