Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5192
|
Name |
pckA
|
Replicon | chromosome |
Genomic location | 5844468 - 5846009 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_253879.1 |
GI | 15600385 |
Affymetrix | PA5192_pckA_at |
Entrez | 879312 |
GenBank | AAG08577.1 |
INSDC | AAG08577.1 |
NCBI Locus Tag | PA5192 |
protein_id(GenBank) | gb|AAG08577.1|AE004932_4|gnl|PseudoCAP|PA5192 |
TIGR | NTL03PA05193 |
UniParc | UPI000013202F |
UniProtKB Acc | Q9HTZ7 |
UniProtKB ID | PCKA_PSEAE |
UniRef100 | UniRef100_Q9HTZ7 |
UniRef50 | UniRef50_Q9HTZ7 |
UniRef90 | UniRef90_Q9HTZ7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
phosphoenolpyruvate carboxykinase
|
Synonyms |
PEPCK PEP carboxykinase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
|
Charge (pH 7) | -12.06 |
Kyte-Doolittle Hydrophobicity Value | -0.152 |
Molecular Weight (kDa) | 55.7 |
Isoelectric Point (pI) | 5.07 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 275 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004776 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5192
Search term: pckA
Search term: phosphoenolpyruvate carboxykinase
|
Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae.
Medina V, Pontarollo R, Glaeske D, Tabel H, Goldie H
J Bacteriol 1990 Dec;172(12):7151-6
PubMed ID: 1701430
|
Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.
Osterås M, Driscoll BT, Finan TM
J Bacteriol 1995 Mar;177(6):1452-60
PubMed ID: 7883700
|
Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase.
Tari LW, Matte A, Pugazhenthi U, Goldie H, Delbaere LT
Nat Struct Biol 1996 Apr;3(4):355-63
PubMed ID: 8599762
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|