Pseudomonas aeruginosa PAO1, PA5217

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5217
Name
Replicon chromosome
Genomic location 5874587 - 5875585 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStructural similarity to 2PT2 (iron-binding protein, FutA1, from Synechocystis 6803, root-mean-square deviation: 0.6 angstroms). Sequence similarity to 6G7N (98%/44,09%) (FE). Final predicted ligand: iron ion (PMID: 31631799)

Cross-References

RefSeq NP_253904.1
GI 15600410
Affymetrix PA5217_at
Entrez 880842
GenBank AAG08602.1
INSDC AAG08602.1
NCBI Locus Tag PA5217
protein_id(GenBank) gb|AAG08602.1|AE004934_9|gnl|PseudoCAP|PA5217
TIGR NTL03PA05218
UniParc UPI00000C5F5B
UniProtKB Acc Q9HTX3
UniProtKB ID P5217_PSEAE
UniRef100 UniRef100_Q9HTX3
UniRef50 UniRef50_Q9HTX3
UniRef90 UniRef90_Q9HTX3

Product

Feature Type CDS
Coding Frame 1
Product Name
putative binding protein of iron ABC transporter
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.86
Kyte-Doolittle Hydrophobicity Value -0.233
Molecular Weight (kDa) 36.3
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 299 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004799 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5217

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequences of the sfuA, sfuB, and sfuC genes of Serratia marcescens suggest a periplasmic-binding-protein-dependent iron transport mechanism.
Angerer A, Gaisser S, Braun V
J Bacteriol 1990 Feb;172(2):572-8
PubMed ID: 2404942
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799