Pseudomonas aeruginosa PAO1, PA5259 (hemD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5259
Name
hemD
Replicon chromosome
Genomic location 5920742 - 5921497 (- strand)

Cross-References

RefSeq NP_253946.1
GI 15600452
Affymetrix PA5259_hemD_at
DNASU PaCD00008267
Entrez 879724
GenBank AAG08644.1
INSDC AAG08644.1
NCBI Locus Tag PA5259
protein_id(GenBank) gb|AAG08644.1|AE004938_6|gnl|PseudoCAP|PA5259
TIGR NTL03PA05260
UniParc UPI000012C4A8
UniProtKB Acc P48246
UniProtKB ID HEM4_PSEAE
UniRef100 UniRef100_P48246
UniRef50 UniRef50_P48246
UniRef90 UniRef90_P48246

Product

Feature Type CDS
Coding Frame 1
Product Name
uroporphyrinogen-III synthetase
Product Name Confidence: Class 2
Synonyms hydroxymethylbilane hydrolyase (cyclizing)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.64
Kyte-Doolittle Hydrophobicity Value -0.053
Molecular Weight (kDa) 27.2
Isoelectric Point (pI) 4.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6TH8 FLAVOPROTEIN 11/19/19 Reconstructing the Origins of the HemD-like fold Thermus thermophilus NOT SOLUTION NMR 94.7
4ES6 LYASE 04/22/12 Crystal structure of HemD (PA5259) from Pseudomonas aeruginosa (PAO1) at 2.22 A resolution Pseudomonas aeruginosa 2.22 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 195 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004840 (518 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5259
Search term: hemD

Human Homologs

References

Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon.
Sasarman A, Nepveu A, Echelard Y, Dymetryszyn J, Drolet M, Goyer C
J Bacteriol 1987 Sep;169(9):4257-62
PubMed ID: 3040684
The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR.
Mohr CD, Sonsteby SK, Deretic V
Mol Gen Genet 1994 Jan;242(2):177-84
PubMed ID: 8159168