Pseudomonas aeruginosa PAO1, PA5277 (lysA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5277
Name
lysA
Replicon chromosome
Genomic location 5941487 - 5942734 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253964.1
GI 15600470
Affymetrix PA5277_lysA_at
DNASU PaCD00008279
Entrez 877749
GenBank AAG08662.1
INSDC AAG08662.1
NCBI Locus Tag PA5277
protein_id(GenBank) gb|AAG08662.1|AE004940_6|gnl|PseudoCAP|PA5277
TIGR NTL03PA05278
UniParc UPI0000128F73
UniProtKB Acc P19572
UniProtKB ID DCDA_PSEAE
UniRef100 UniRef100_P19572
UniRef50 UniRef50_P19572
UniRef90 UniRef90_P19572

Product

Feature Type CDS
Coding Frame 1
Product Name
diaminopimelate decarboxylase
Product Name Confidence: Class 1
Synonyms DAP decarboxylase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.45
Kyte-Doolittle Hydrophobicity Value -0.120
Molecular Weight (kDa) 45.5
Isoelectric Point (pI) 5.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004855 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5277
Search term: lysA

Human Homologs

References

Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli.
Martin C, Cami B, Borne F, Jeenes DJ, Haas D, Patte JC
Mol Gen Genet 1986 Jun;203(3):430-4
PubMed ID: 3018430
Pseudomonas aeruginosa diaminopimelate decarboxylase: evolutionary relationship with other amino acid decarboxylases.
Martin C, Cami B, Yeh P, Stragier P, Parsot C, Patte JC
Mol Biol Evol 1988 Sep;5(5):549-59
PubMed ID: 3143046
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994