Pseudomonas aeruginosa PAO1, PA5278 (dapF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5278
Name
dapF
Replicon chromosome
Genomic location 5942745 - 5943575 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253965.1
GI 15600471
Affymetrix PA5278_dapF_at
DNASU PaCD00008280
Entrez 877750
GenBank AAG08663.1
INSDC CAA55224.1
NCBI Locus Tag PA5278
protein_id(GenBank) gb|AAG08663.1|AE004940_7|gnl|PseudoCAP|PA5278
TIGR NTL03PA05279
UniParc UPI0000128EBE
UniProtKB Acc Q51564
UniProtKB ID DAPF_PSEAE
UniRef100 UniRef100_B7V5G9
UniRef50 UniRef50_Q88CF3
UniRef90 UniRef90_B7V5G9

Product

Feature Type CDS
Coding Frame 1
Product Name
diaminopimelate epimerase
Product Name Confidence: Class 2
Synonyms DAP epimerase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.28
Kyte-Doolittle Hydrophobicity Value -0.212
Molecular Weight (kDa) 30.3
Isoelectric Point (pI) 7.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 553 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004856 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5278
Search term: dapF

Human Homologs

References

Nucleotide sequence of the dapF gene and flanking regions from Escherichia coli K12.
Richaud C, Printz C
Nucleic Acids Res 1988 Nov 11;16(21):10367
PubMed ID: 3057443
Expression of recombinant diaminopimelate epimerase in Escherichia coli. Isolation and inhibition with an irreversible inhibitor.
Higgins W, Tardif C, Richaud C, Krivanek MA, Cardin A
Eur J Biochem 1989 Dec 8;186(1-2):137-43
PubMed ID: 2689171
A site-specific recombinase is required for competitive root colonization by Pseudomonas fluorescens WCS365.
Dekkers LC, Phoelich CC, van der Fits L, Lugtenberg BJ
Proc Natl Acad Sci U S A 1998 Jun 9;95(12):7051-6
PubMed ID: 9618537