Pseudomonas aeruginosa PAO1, PA5296 (rep)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5296
Name
rep
Replicon chromosome
Genomic location 5962716 - 5964725 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253983.1
GI 15600489
Affymetrix PA5296_rep_at
Entrez 881391
GenBank AAG08681.1
INSDC AAG08681.1
NCBI Locus Tag PA5296
protein_id(GenBank) gb|AAG08681.1|AE004942_4|gnl|PseudoCAP|PA5296
TIGR NTL03PA05297
UniParc UPI00000C5F92
UniProtKB Acc Q9HTQ8
UniProtKB ID Q9HTQ8_PSEAE
UniRef100 UniRef100_W0Z068
UniRef50 UniRef50_P09980
UniRef90 UniRef90_A6VEA1

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP-dependent DNA helicase Rep
Product Name Confidence: Class 2
Synonyms Rep helicase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.76
Kyte-Doolittle Hydrophobicity Value -0.462
Molecular Weight (kDa) 77.0
Isoelectric Point (pI) 5.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 638 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004871 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5296
Search term: rep

Human Homologs

References

Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein.
Bialkowska-Hobrzanska H, Gilchrist CA, Denhardt DT
J Bacteriol 1985 Dec;164(3):1004-10
PubMed ID: 2999067
Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP.
Korolev S, Hsieh J, Gauss GH, Lohman TM, Waksman G
Cell 1997 Aug 22;90(4):635-47
PubMed ID: 9288744