Pseudomonas aeruginosa PAO1, PA5297 (poxB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5297
Name
poxB
Replicon chromosome
Genomic location 5964859 - 5966577 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_253984.1
GI 15600490
Affymetrix PA5297_poxB_at
Entrez 881392
GenBank AAG08682.1
INSDC AAG08682.1
NCBI Locus Tag PA5297
protein_id(GenBank) gb|AAG08682.1|AE004942_5|gnl|PseudoCAP|PA5297
TIGR NTL03PA05298
UniParc UPI00000D74B4
UniProtKB Acc Q9HTQ7
UniProtKB ID Q9HTQ7_PSEAE
UniRef100 UniRef100_Q9HTQ7
UniRef50 UniRef50_B2FRQ8
UniRef90 UniRef90_A0A077JYZ4

Product

Feature Type CDS
Coding Frame 1
Product Name
pyruvate dehydrogenase (cytochrome)
Product Name Confidence: Class 2
Synonyms pyruvate oxidase
Evidence for Translation
Charge (pH 7) -12.71
Kyte-Doolittle Hydrophobicity Value 0.006
Molecular Weight (kDa) 62.3
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 594 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003359 (768 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5297
Search term: poxB

Human Homologs

Ensembl 110, assembly GRCh38.p14
2-hydroxyacyl-CoA lyase 1 [Source:HGNC Symbol;Acc:HGNC:17856]
E-value: 2.3e-21
Percent Identity: 22.7

References

Nucleotide sequence and deduced amino acid sequence of Escherichia coli pyruvate oxidase, a lipid-activated flavoprotein.
Grabau C, Cronan JE Jr
Nucleic Acids Res 1986 Jul 11;14(13):5449-60
PubMed ID: 3016647
Lipid binding by Escherichia coli pyruvate oxidase is disrupted by small alterations of the carboxyl-terminal region.
Grabau C, Chang YY, Cronan JE Jr
J Biol Chem 1989 Jul 25;264(21):12510-9
PubMed ID: 2663858