Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5302
|
Name |
dadX
Synonym: alr2 |
Replicon | chromosome |
Genomic location | 5970686 - 5971759 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 6 transposon mutants in orthologs |
RefSeq | NP_253989.1 |
GI | 15600495 |
Affymetrix | PA5302_dadX_at |
Entrez | 878055 |
GenBank | AAG08687.1 |
INSDC | AAG08687.1 |
NCBI Locus Tag | PA5302 |
protein_id(GenBank) | gb|AAG08687.1|AE004943_3|gnl|PseudoCAP|PA5302 |
TIGR | NTL03PA05303 |
UniParc | UPI00001258D4 |
UniProtKB Acc | Q9HTQ2 |
UniProtKB ID | ALR2_PSEAE |
UniRef100 | UniRef100_Q9HTQ2 |
UniRef50 | UniRef50_Q9HTQ2 |
UniRef90 | UniRef90_Q9HTQ2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
catabolic alanine racemase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -0.77 |
Kyte-Doolittle Hydrophobicity Value | 0.005 |
Molecular Weight (kDa) | 38.9 |
Isoelectric Point (pI) | 6.85 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1RCQ | ISOMERASE | 11/04/03 | The 1.45 A crystal structure of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms | Pseudomonas aeruginosa | 1.45 | X-RAY DIFFRACTION | 99.2 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
D-Lysine | DB03252 | DrugBank | 5.1.4 | Q9HTQ2 | Alanine racemase, catabolic | 100.0 | 357 | 1.4e-205 |
Lysine Nz-Carboxylic Acid | DB03801 | DrugBank | 5.1.4 | Q9HTQ2 | Alanine racemase, catabolic | 100.0 | 357 | 1.4e-205 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 560 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004545 (850 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5302
Search term: dadX
Search term: catabolic alanine racemase
|
Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase.
Lobocka M, Hennig J, Wild J, KÅ‚opotowski T
J. Bacteriol. 1994 Mar;176(5):1500-10
PubMed ID: 7906689
|
Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.
Strych U, Huang HC, Krause KL, Benedik MJ
Curr Microbiol 2000 Oct;41(4):290-4
PubMed ID: 10977898
|