Pseudomonas aeruginosa PAO1, PA5321 (dut)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5321
Name
dut
Replicon chromosome
Genomic location 5990676 - 5991131 (+ strand)

Cross-References

RefSeq NP_254008.1
GI 15600514
Affymetrix PA5321_dut_at
DNASU PaCD00008298
Entrez 879405
GenBank AAG08706.1
INSDC AAG08706.1
NCBI Locus Tag PA5321
protein_id(GenBank) gb|AAG08706.1|AE004944_10|gnl|PseudoCAP|PA5321
TIGR NTL03PA05322
UniParc UPI0000129990
UniProtKB Acc Q9HTN3
UniProtKB ID DUT_PSEAE
UniRef100 UniRef100_B7V5L2
UniRef50 UniRef50_B7NQ01
UniRef90 UniRef90_A6VEC8

Product

Feature Type CDS
Coding Frame 1
Product Name
deoxyuridine 5'-triphosphate nucleotidohydrolase
Product Name Confidence: Class 2
Synonyms dUTPase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -4.60
Kyte-Doolittle Hydrophobicity Value 0.175
Molecular Weight (kDa) 15.9
Isoelectric Point (pI) 5.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 476 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004892 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5321
Search term: dut

Human Homologs

References

Crystal structure of a dUTPase.
Cedergren-Zeppezauer ES, Larsson G, Nyman PO, Dauter Z, Wilson KS
Nature 1992 Feb 20;355(6362):740-3
PubMed ID: 1311056
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142