Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5322
|
Name |
algC
Synonym: pmm |
Replicon | chromosome |
Genomic location | 5991168 - 5993774 (+ strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_254009 |
GI | 15600515 |
Affymetrix | PA5322_algC_at |
Entrez | 879406 |
GenBank | AAG08707.1 |
NCBI Locus Tag | PA5322 |
protein_id(GenBank) | gb|AAG08707.1|AE004945_1|gnl|PseudoCAP|PA5322 |
TIGR | NTL03PA05323 |
UniProtKB Acc | P26276 |
UniProtKB ID | ALGC_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
phosphomannomutase AlgC
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -10.75 |
Kyte-Doolittle Hydrophobicity Value | -0.065 |
Molecular Weight (kDa) | 50.3 |
Isoelectric Point (pI) | 4.95 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1P5D | ISOMERASE | 04/25/03 | Enzyme-ligand complex of P. aeruginosa PMM/PGM | Pseudomonas aeruginosa | 1.6 | X-RAY DIFFRACTION | 100.0 |
1PCM | ISOMERASE | 05/16/03 | Enzyme-ligand complex of P. aeruginosa PMM/PGM | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
1K35 | ISOMERASE | 10/01/01 | Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 100.0 |
2H4L | ISOMERASE | 05/24/06 | Complex of PMM/PGM with ribose 1-phosphate | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 99.8 |
2FKF | ISOMERASE | 01/04/06 | Phosphomannomutase/Phosphoglucomutase from Pseudomonas aeruginosa with alpha-D-glucose 1,6-bisphosphate bound | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
3BKQ | ISOMERASE | 12/07/07 | Structure of the P368G mutant of PMM/PGM in complex with its substrate | Pseudomonas aeruginosa | 2.05 | X-RAY DIFFRACTION | 99.8 |
4IL8 | ISOMERASE | 12/29/12 | Crystal structure of an H329A mutant of p. aeruginosa PMM/PGM | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 99.6 |
3C04 | ISOMERASE | 01/18/08 | Structure of the P368G mutant of PMM/PGM from P. aeruginosa | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 99.8 |
1P5G | ISOMERASE | 04/26/03 | Enzyme-ligand complex of P. aeruginosa PMM/PGM | Pseudomonas aeruginosa | 1.61 | X-RAY DIFFRACTION | 100.0 |
3RSM | ISOMERASE | 05/02/11 | Crystal structure of S108C mutant of PMM/PGM | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 99.6 |
2H5A | ISOMERASE | 05/25/06 | Complex of the enzyme PMM/PGM with xylose 1-phosphate | Pseudomonas aeruginosa | 1.72 | X-RAY DIFFRACTION | 99.8 |
4MRQ | ISOMERASE | 09/17/13 | Crystal Structure of wild-type unphosphorylated PMM/PGM | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
1K2Y | ISOMERASE | 09/30/01 | Crystal Structure of Phosphomannomutase/Phosphoglucomutase S108A mutant from P. aeruginosa | Pseudomonas aeruginosa | 1.75 | X-RAY DIFFRACTION | 99.8 |
2FKM | ISOMERASE | 01/04/06 | PMM/PGM S108D mutant with alpha-d-glucose 1,6-bisphosphate bound | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 99.8 |
1PCJ | ISOMERASE | 05/16/03 | Enzyme-ligand complex of P. aeruginosa PMM/PGM | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Dexfosfoserine | DB04522 | DrugBank | 5.1.4 | P26276 | Phosphomannomutase/phosphoglucomutase | 100.0 | 463 | 7.4e-271 |
alpha-D-glucose-1-phosphate | DB02843 | DrugBank | 5.1.4 | P26276 | Phosphomannomutase/phosphoglucomutase | 100.0 | 463 | 7.4e-271 |
alpha-D-glucose 6-phosphate | DB02007 | DrugBank | 5.1.4 | P26276 | Phosphomannomutase/phosphoglucomutase | 100.0 | 463 | 7.4e-271 |
D-Mannose 1-Phosphate | DB02867 | DrugBank | 5.1.4 | P26276 | Phosphomannomutase/phosphoglucomutase | 100.0 | 463 | 7.4e-271 |
alpha-D-mannose 6-phosphate | DB02900 | DrugBank | 5.1.4 | P26276 | Phosphomannomutase/phosphoglucomutase | 100.0 | 463 | 7.4e-271 |
Source 1 | |
Database | PseudoCAP |
Category | Alginate biosynthesis |
Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
Host Organism | Mus musculus |
Notes | |
Infection model | Mouse burn Infection model |
Reference | 7558335 |
Source 2 | |
Database | Victors : 4229 |
Category | Alginate biosynthesis |
Evidence |
ECO:0000015
mutant phenotype evidence
|
Host Organism | Mus musculus |
Notes | |
Reference | 8557368 |
Source 3 | |
Database | VFDB |
Category | Alginate biosynthesis |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 660 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004893 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5322
Search term: algC
Search term: phosphomannomutase AlgC
|
The Pseudomonas aeruginosa algC gene product participates in rhamnolipid biosynthesis.
Olvera C, Goldberg JB, Sánchez R, Soberón-Chávez G
FEMS Microbiol Lett 1999 Oct 1;179(1):85-90
PubMed ID: 10481091
|
Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase.
Zielinski NA, Chakrabarty AM, Berry A
J Biol Chem 1991 May 25;266(15):9754-63
PubMed ID: 1903398
|
Alginate synthesis in Pseudomonas aeruginosa: environmental regulation of the algC promoter.
Zielinski NA, Maharaj R, Roychoudhury S, Danganan CE, Hendrickson W, Chakrabarty AM
J Bacteriol 1992 Dec;174(23):7680-8
PubMed ID: 1447138
|
Purification and characterization of phosphomannomutase/phosphoglucomutase from Pseudomonas aeruginosa involved in biosynthesis of both alginate and lipopolysaccharide.
Ye RW, Zielinski NA, Chakrabarty AM
J Bacteriol 1994 Aug;176(16):4851-7
PubMed ID: 8050998
|
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602
|