Pseudomonas aeruginosa PAO1, PA5322 (algC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5322
Name
algC
Synonym: pmm
Replicon chromosome
Genomic location 5991168 - 5993774 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_254009
GI 15600515
Affymetrix PA5322_algC_at
Entrez 879406
GenBank AAG08707.1
NCBI Locus Tag PA5322
protein_id(GenBank) gb|AAG08707.1|AE004945_1|gnl|PseudoCAP|PA5322
TIGR NTL03PA05323
UniProtKB Acc P26276
UniProtKB ID ALGC_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphomannomutase AlgC
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.75
Kyte-Doolittle Hydrophobicity Value -0.065
Molecular Weight (kDa) 50.3
Isoelectric Point (pI) 4.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2H5A ISOMERASE 05/25/06 Complex of the enzyme PMM/PGM with xylose 1-phosphate PSEUDOMONAS AERUGINOSA 1.72 X-RAY DIFFRACTION 99.8
1PCM ISOMERASE 05/16/03 Enzyme-ligand complex of P. aeruginosa PMM/PGM PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
3BKQ ISOMERASE 12/07/07 Structure of the P368G mutant of PMM/PGM in complex with its substrate PSEUDOMONAS AERUGINOSA 2.05 X-RAY DIFFRACTION 99.8
1K35 ISOMERASE 10/01/01 Crystal Structure of Phosphomannomutase/Phosphoglucomutase from P.aeruginosa PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0
1P5D ISOMERASE 04/25/03 Enzyme-ligand complex of P. aeruginosa PMM/PGM PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 100.0
4MRQ ISOMERASE 09/17/13 Crystal Structure of wild-type unphosphorylated PMM/PGM PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
3C04 ISOMERASE 01/18/08 Structure of the P368G mutant of PMM/PGM from P. aeruginosa PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 99.8
1P5G ISOMERASE 04/26/03 Enzyme-ligand complex of P. aeruginosa PMM/PGM PSEUDOMONAS AERUGINOSA 1.61 X-RAY DIFFRACTION 100.0
1K2Y ISOMERASE 09/30/01 Crystal Structure of Phosphomannomutase/Phosphoglucomutase S108A mutant from P. aeruginosa PSEUDOMONAS AERUGINOSA 1.75 X-RAY DIFFRACTION 99.8
4IL8 ISOMERASE 12/29/12 Crystal structure of an H329A mutant of p. aeruginosa PMM/PGM PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.6
2FKF ISOMERASE 01/04/06 Phosphomannomutase/Phosphoglucomutase from Pseudomonas aeruginosa with alpha-D-glucose 1,6-bisphosphate bound PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
2H4L ISOMERASE 05/24/06 Complex of PMM/PGM with ribose 1-phosphate PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 99.8
3RSM ISOMERASE 05/02/11 Crystal structure of S108C mutant of PMM/PGM PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 99.6
1PCJ ISOMERASE 05/16/03 Enzyme-ligand complex of P. aeruginosa PMM/PGM PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
2FKM ISOMERASE 01/04/06 PMM/PGM S108D mutant with alpha-d-glucose 1,6-bisphosphate bound PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 99.8

Virulence Evidence

Source 1
Database Victors : 4229
Category Alginate biosynthesis
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 8557368
Source 2
Database VFDB
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference
Source 3
Database PseudoCAP
Category Alginate biosynthesis
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse burn Infection model
Reference 7558335

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 660 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004893 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5322
Search term: algC

References

The Pseudomonas aeruginosa algC gene product participates in rhamnolipid biosynthesis.
Olvera C, Goldberg JB, Sánchez R, Soberón-Chávez G
FEMS Microbiol. Lett. 1999 Oct;179(1):85-90
PubMed ID: 10481091
Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase.
Zielinski NA, Chakrabarty AM, Berry A
J. Biol. Chem. 1991 May;266(15):9754-63
PubMed ID: 1903398
Alginate synthesis in Pseudomonas aeruginosa: environmental regulation of the algC promoter.
Zielinski NA, Maharaj R, Roychoudhury S, Danganan CE, Hendrickson W, Chakrabarty AM
J. Bacteriol. 1992 Dec;174(23):7680-8
PubMed ID: 1447138
Purification and characterization of phosphomannomutase/phosphoglucomutase from Pseudomonas aeruginosa involved in biosynthesis of both alginate and lipopolysaccharide.
Ye RW, Zielinski NA, Chakrabarty AM
J. Bacteriol. 1994 Aug;176(16):4851-7
PubMed ID: 8050998
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan;96
PubMed ID: 24291602