Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5334
|
Name |
rph
|
Replicon | chromosome |
Genomic location | 6003377 - 6004096 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
RefSeq | NP_254021.1 |
GI | 15600527 |
Affymetrix | PA5334_rph_at |
DNASU | PaCD00008305 |
Entrez | 878218 |
GenBank | AAG08719.1 |
INSDC | AAG08719.1 |
NCBI Locus Tag | PA5334 |
protein_id(GenBank) | gb|AAG08719.1|AE004946_3|gnl|PseudoCAP|PA5334 |
TIGR | NTL03PA05335 |
UniParc | UPI0000134463 |
UniProtKB Acc | P50597 |
UniProtKB ID | RNPH_PSEAE |
UniRef100 | UniRef100_B7V5M5 |
UniRef50 | UniRef50_P28619 |
UniRef90 | UniRef90_A6VEE1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
ribonuclease PH
|
Synonyms |
tRNA nucleotidyltransferase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -1.58 |
Kyte-Doolittle Hydrophobicity Value | -0.183 |
Molecular Weight (kDa) | 25.7 |
Isoelectric Point (pI) | 5.89 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1R6M | TRANSFERASE | 10/15/03 | Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
1R6L | TRANSFERASE | 10/15/03 | Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
N-cyclohexyltaurine | DB03309 | DrugBank | 5.1.4 | P50597 | Ribonuclease PH | 100.0 | 239 | 1.8e-127 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 506 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004903 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5334
Search term: rph
Search term: ribonuclease PH
|
Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli.
Jensen KF, Andersen JT, Poulsen P
J Biol Chem 1992 Aug 25;267(24):17147-52
PubMed ID: 1512252
|
Characterization of Escherichia coli RNase PH.
Kelly KO, Deutscher MP
J Biol Chem 1992 Aug 25;267(24):17153-8
PubMed ID: 1512253
|