Pseudomonas aeruginosa PAO1, PA5334 (rph)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5334
Name
rph
Replicon chromosome
Genomic location 6003377 - 6004096 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_254021.1
GI 15600527
Affymetrix PA5334_rph_at
DNASU PaCD00008305
Entrez 878218
GenBank AAG08719.1
INSDC AAG08719.1
NCBI Locus Tag PA5334
protein_id(GenBank) gb|AAG08719.1|AE004946_3|gnl|PseudoCAP|PA5334
TIGR NTL03PA05335
UniParc UPI0000134463
UniProtKB Acc P50597
UniProtKB ID RNPH_PSEAE
UniRef100 UniRef100_B7V5M5
UniRef50 UniRef50_P28619
UniRef90 UniRef90_A6VEE1

Product

Feature Type CDS
Coding Frame 1
Product Name
ribonuclease PH
Product Name Confidence: Class 2
Synonyms tRNA nucleotidyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.58
Kyte-Doolittle Hydrophobicity Value -0.183
Molecular Weight (kDa) 25.7
Isoelectric Point (pI) 5.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1R6M TRANSFERASE 10/15/03 Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0
1R6L TRANSFERASE 10/15/03 Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-cyclohexyltaurine DB03309 DrugBank 5.1.4 P50597 Ribonuclease PH 100.0 239 1.8e-127

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 506 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004903 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5334
Search term: rph
Search term: ribonuclease PH

Human Homologs

References

Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli.
Jensen KF, Andersen JT, Poulsen P
J Biol Chem 1992 Aug 25;267(24):17147-52
PubMed ID: 1512252
Characterization of Escherichia coli RNase PH.
Kelly KO, Deutscher MP
J Biol Chem 1992 Aug 25;267(24):17153-8
PubMed ID: 1512253