Pseudomonas aeruginosa PAO1, PA5345 (recG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5345
Name
recG
Replicon chromosome
Genomic location 6012976 - 6015051 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254032.1
GI 15600538
Affymetrix PA5345_recG_at
Entrez 880937
GenBank AAG08730.1
INSDC AAG08730.1
NCBI Locus Tag PA5345
protein_id(GenBank) gb|AAG08730.1|AE004946_14|gnl|PseudoCAP|PA5345
TIGR NTL03PA05346
UniParc UPI00000C5FB5
UniProtKB Acc Q9HTL3
UniProtKB ID Q9HTL3_PSEAE
UniRef100 UniRef100_Q9HTL3
UniRef50 UniRef50_Q9HTL3
UniRef90 UniRef90_Q9HTL3

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP-dependent DNA helicase RecG
Product Name Confidence: Class 1
Synonyms RecG protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.19
Kyte-Doolittle Hydrophobicity Value -0.128
Molecular Weight (kDa) 76.2
Isoelectric Point (pI) 7.31

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004913 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5345
Search term: recG

Human Homologs

References

Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12.
Lloyd RG, Sharples GJ
J Bacteriol 1991 Nov;173(21):6837-43
PubMed ID: 1938888
Escherichia coli RecG and RecA proteins in R-loop formation.
Hong X, Cadwell GW, Kogoma T
EMBO J 1995 May 15;14(10):2385-92
PubMed ID: 7774596
Involvement of DNA helicases in chromate resistance by Pseudomonas aeruginosa PAO1.
Miranda AT, González MV, González G, Vargas E, Campos-García J, Cervantes C
Mutat Res 2005 Oct 15;578(1-2):202-9
PubMed ID: 16105671