Pseudomonas aeruginosa PAO1, PA5349 (rubB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5349
Name
rubB
Replicon chromosome
Genomic location 6017624 - 6018778 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254036.1
GI 15600542
Affymetrix PA5349_at
DNASU PaCD00008315
Entrez 879643
GenBank AAG08734.1
INSDC AAG08734.1
NCBI Locus Tag PA5349
protein_id(GenBank) gb|AAG08734.1|AE004947_4|gnl|PseudoCAP|PA5349
TIGR NTL03PA05350
UniParc UPI00000C5FB8
UniProtKB Acc Q9HTK9
UniProtKB ID RURE_PSEAE
UniRef100 UniRef100_Q9HTK9
UniRef50 UniRef50_Q9HTK9
UniRef90 UniRef90_Q9HTK9

Product

Feature Type CDS
Coding Frame 1
Product Name
rubredoxin reductase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.76
Kyte-Doolittle Hydrophobicity Value 0.087
Molecular Weight (kDa) 40.6
Isoelectric Point (pI) 5.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2V3A OXIDOREDUCTASE 06/14/07 Crystal structure of rubredoxin reductase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
2V3B OXIDOREDUCTASE 06/14/07 Crystal structure of the electron transfer complex rubredoxin - rubredoxin reductase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.45 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 601 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004917 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5349
Search term: rubB

Human Homologs

References

Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1.
Haspel G, Ehrt S, Hillen W
Microbiology (Reading) 1995 Jun;141 ( Pt 6):1425-1432
PubMed ID: 7670642
Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa.
Smits TH, Witholt B, van Beilen JB
Antonie Van Leeuwenhoek 2003;84(3):193-200
PubMed ID: 14574114