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Pseudomonas Genome Database - Annotation for PA1777
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               gene PA1777                
Key to diagram


View in GBrowse: 22 putative orthologs Acknowledgements for this annotation
   
Feature Overview
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Pseudomonas aeruginosa PAO1 chromosome, complete genome
(5677 genes, 6264404 bp) [ RefSeq ]
Feature type gene  Genomic location 1921174 - 1922226 (+ strand)
Name oprF  Alternate Names
Locus tag PA1777 (View 10 updates) Submit an Update Tranposon Mutant Available? 2 mutant(s) available.
Product Information
Feature type CDS
Product Name Major porin and structural outer membrane porin OprF precursor (Class 1)
Alternate Names Porin F

Proteomics/Comparative Genomics
Subcellular localization
Outer Membrane (Class 1Explanation of Localization Confidence)
Pseudomonas Ortholog Group Explanation of Orthologous Groups
POG003618 
Orthology uncertain because more than one top BLASTp hit was identified.
Number of Orthologs 22 putative orthologs
Secondary Genomic Features contained in PA1777
Features
Type Name Sequence Start Stop Strand PubMed IDs External DB
transposon_insertion_site ISphoA/hah insertion site
 1921297 1921297 + 14617778 UWGC: 31899
transposon_insertion_site Confirmed ISlacZ/hah insertion site
 1921605 1921605 + 14617778 UWGC: 14868
Alternate Annotations and Cross-References
GI 15596974
Precomputed BLAST results NCBI BLINK
RefSeq NP_250468
TIGR NTL03PA01778
UniProtKB Acc P13794
UniProtKB ID PORF_PSEAE
KEGG PA1777
GBrowse Click GBrowse for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Membrane proteins
Transport of small molecules
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0006810 transport IDA
Cellular Component
GO:0016020 membrane IDA
GO:0009279 cell outer membrane IEA
COG PredictionExplanation of COG predictions
Accession Description Category
COG2885 Outer membrane protein and related peptidoglycan-associated (lipo)proteins [Cell envelope biogenesis, outer membrane]
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 100% similar to PA porin F protein
Structure Features
Structure Features Explanation of Structure Features Field PS01068: OmpA-like domain
type I export signal computationally predicted by LipoP v.1.0 (cleavage after residue 24)
type I export signal computationally predicted by Phobius (cleavage after residue 24)
type I export signal computationally predicted by SignalP v.3.0 Hidden Markov Models (cleavage after residue 24)
CDD predictions Explanation of CDD predictions
CDD Accession External Accession Database Short Name Description Start Stop E-value Release Number
114461 pfam05736 Pfam OprF OprF membrane domain 1 188 2e-83 2.29
190797 pfam03922 Pfam OmpW OmpW family 54 184 8e-06 2.29
182748 PRK10808 PRK PRK10808 outer membrane protein A; Reviewed 142 346 9e-17 2.29
32711 COG2885 COG OmpA Outer membrane protein and related peptidoglycan-associated (lipo)proteins [Cell envelope biogenesis, outer membrane] 174 345 8e-33 2.29
182744 PRK10802 PRK PRK10802 peptidoglycan-associated outer membrane lipoprotein; Provisional 200 340 7e-12 2.29
180801 PRK07033 PRK PRK07033 hypothetical protein; Provisional 235 342 2e-13 2.29
180754 PRK06925 PRK PRK06925 flagellar motor protein MotS; Reviewed 235 342 1e-10 2.29
180652 PRK06667 PRK motB flagellar motor protein MotB; Validated 235 342 3e-11 2.29
143586 cd07185 CD OmpA_C-like Peptidoglycan binding domains similar to the C-terminal domain of outer-membrane protein OmpA 239 345 2e-37 2.29
181095 PRK07734 PRK motB flagellar motor protein MotB; Reviewed 241 342 1e-11 2.29
182507 PRK10510 PRK PRK10510 putative outer membrane lipoprotein; Provisional 241 342 6e-16 2.29
181435 PRK08457 PRK motB flagellar motor protein MotB; Reviewed 241 342 5e-09 2.29
188250 TIGR02802 TIGRFAM Pal_lipo peptidoglycan-associated lipoprotein 242 342 4e-22 2.29
102525 PRK06742 PRK PRK06742 flagellar motor protein MotS; Reviewed 244 341 1e-08 2.29
189673 pfam00691 Pfam OmpA OmpA family 244 339 2e-19 2.29
182172 PRK09967 PRK PRK09967 putative outer membrane lipoprotein; Provisional 244 341 4e-13 2.29
181618 PRK09038 PRK PRK09038 flagellar motor protein MotD; Reviewed 254 341 2e-16 2.29
181590 PRK08944 PRK motB flagellar motor protein MotB; Reviewed 259 342 3e-12 2.29
181240 PRK08126 PRK PRK08126 hypothetical protein; Provisional 275 345 1e-15 2.29
181619 PRK09039 PRK PRK09039 hypothetical protein; Validated 276 342 1e-09 2.29
168775 PRK07034 PRK PRK07034 hypothetical protein; Provisional 276 342 3e-06 2.29
181620 PRK09040 PRK PRK09040 hypothetical protein; Provisional 287 342 7e-08 2.29
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GAGGTGATGCTGAAAGTTTTGTACGGGTTGAAGAACTTTGAGGGCAAGTCGAAGTTCAAAACGTGGCTATATAGCATCACGTACAACGAGTGCATCACGC
AGTACCGCAAGGAGCGCCGCAAGCGCCGATTGATGGATGCGCTCAGCCTCGACCCGCTGGAGGAGGCTTCCGAGGAGAAGTCGCCGAAGGTTGAGGAGCG
GGGCGGATTGGATCGTTGGCTCGTCCATGTCAATCCGATAGATCGGGAGATTCTGGTTCTTCGCTTCGTCGCGGAACTGGAGTTTCAGGAGATAGCGGAT
ATCATGCACATGGGTCTGAGTGCGACGAAAATGCGCTACAAGCGGGCACTGGACCGCCTGCGCGAAAAGTTTTCAGATGCGACCGAAACATAGTTGGGTA
AATATTGTCTCTCTATGCGGGAAGTTCTGATAAACTTGCCACCCAAGTTGTGCGGCTGATTGTTGGACAACTAACTGACCATCAAGATGGGGATTTAACG
G

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAAACTGAAGAACACCTTAGGCGTTGTCATCGGCTCGCTGGTTGCCGCTTCGGCAATGAACGCCTTTGCCCAGGGCCAGAACTCGGTAGAGATCGAAG
CCTTCGGCAAGCGCTACTTCACCGACAGCGTTCGCAACATGAAGAACGCGGACCTGTACGGCGGCTCGATCGGTTACTTCCTGACCGACGACGTCGAGCT
GGCGCTGTCCTACGGTGAGTACCATGACGTTCGTGGCACCTACGAAACCGGCAACAAGAAGGTCCACGGCAACCTGACCTCCCTGGACGCCATCTACCAC
TTCGGTACCCCGGGCGTAGGTCTGCGTCCGTACGTGTCGGCTGGTCTGGCTCACCAGAACATCACCAACATCAACAGCGACAGCCAAGGCCGTCAGCAGA
TGACCATGGCCAACATCGGCGCTGGTCTGAAGTACTACTTCACCGAGAACTTCTTCGCCAAGGCCAGCCTCGACGGCCAGTACGGTCTGGAGAAGCGTGA
CAACGGTCACCAGGGCGAGTGGATGGCTGGCCTGGGCGTCGGCTTCAACTTCGGTGGTTCGAAAGCCGCTCCGGCTCCGGAACCGGTTGCCGACGTTTGC
TCCGACTCCGACAACGACGGCGTTTGCGACAACGTCGACAAGTGCCCGGATACCCCGGCCAACGTCACCGTTGACGCCAACGGCTGCCCGGCTGTCGCCG
AAGTCGTACGCGTACAGCTGGACGTGAAGTTCGACTTCGACAAGTCCAAGGTCAAAGAGAACAGCTACGCTGACATCAAGAACCTGGCTGACTTCATGAA
GCAGTACCCGTCCACTTCCACCACCGTTGAAGGTCACACCGACTCCGTCGGCACCGACGCTTACAACCAGAAGCTGTCCGAGCGTCGTGCCAACGCCGTT
CGTGACGTACTGGTCAACGAGTACGGTGTAGAAGGTGGTCGCGTGAACGCTGTTGGTTACGGCGAGTCCCGCCCGGTTGCCGACAACGCCACCGCTGAAG
GCCGCGCTATCAACCGTCGCGTTGAAGCCGAAGTAGAAGCTGAAGCCAAGTAA

BLAST search against this genome or other genomes in database

Downstream 500 BP region TCGGCTGAGCCTCTAAGGAAAAACCCGGCCCAGGCCGGGTTTTTCTTTGCCTGGAAAAAGACCGCTCGTCAGGCGCTCAGGGAAATCGGTTGCGACACGA
TGTCGCGGGTTACTTCGCCGATCACCAGGATCGCCGGGCTTTTCAAGGCGAAACGTCCGGCGTCCCGCAGTAGCTCGCCGAGGTTGCTGCGGCATTCGCG
CTGGTTGCCCAGGGTGGCGTTCTCGATCATCGCCAGCGGCGTATCCTCGGCCATACCTCCAGCCAGCAGCCCGGCCTGGATCTCTGCGAGACGGGCGACG
CCCATGTAGACCACCAGCGTGGTACCGCTGCGTGCCAGGGCTTCCCAGGCCAGCGGGCTGTCGTCCTGGGTATGCGCGGTCACCAGGGTCACGCCGCGGC
TGATGCCGCGGTAGGTCAGCGGTATGCCGCAGGCAGTGGCGCCAGCCAGCCCGGCGGTGATGCCGTTCACTATCTCGCTGTCTATCCCGTGCCGGGCCAG
C

Protein Sequence MKLKNTLGVVIGSLVAASAMNAFAQGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALSYGEYHDVRGTYETGNKKVHGNLTSLDAIYH
FGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIGAGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVC
SDSDNDGVCDNVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHTDSVGTDAYNQKLSERRANAV
RDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK*

BLAST search against this genome or other genomes in database
Precomputed BLAST results at BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.
Showing SNPs occurring in PA1777 gene of Pseudomonas aeruginosa PAO1, Pseudomonas aeruginosa PAO1 chromosome, complete genome (1921174 to 1922226 bp)
  • Showing SNPs in Pseudomonas aeruginosa PACS2
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PACS2 Transition g.1922124T>C
Silent
GCT(Ala) -> GCC(Ala) 		SNP136-1922124-112 View
Select to view SNPs in a specific strain
SNP Map
Sequences
DNAProtein
Orthologous sequences (strains of same species) Download/Align Download/Align
Orthologous sequences (all strains/species) Download/Align Download/Align
Operons/Regulons
No operon/regulon data available
PubMed Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S.
J Proteome Res. 2005 Nov-Dec;4(6):2257-65.
PMID: 16335974
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.
Duchene M, Schweizer A, Lottspeich F, Krauss G, Marget M, Vogel K, von Specht BU, Domdey H.
J Bacteriol. 1988 Jan;170(1):155-62.
PMID: 2447060
Full virulence of Pseudomonas aeruginosa requires OprF.
Fito-Boncompte L, Chapalain A, Bouffartigues E, Chaker H, Lesouhaitier O, Gicquel G, Bazire A, Madi A, Connil N, Veron W, Taupin L, Toussaint B, Cornelis P, Wei Q, Shioya K, Deziel E, Feuilloley MG, Orange N, Dufour A, Chevalier S.
Infect Immun. 2011 Mar;79(3):1176-86. Epub 2010 Dec 28.
PMID: 21189321
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2011; PMID:20929876) and not the web site URL. Thank you!

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